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首页> 外文期刊>Clinical and Experimental Immunology: An Official Journal of the British Society for Immunology >Antibody responses to deamidated gliadin peptide show high specificity and parallel antibodies to tissue transglutaminase in developing coeliac disease.
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Antibody responses to deamidated gliadin peptide show high specificity and parallel antibodies to tissue transglutaminase in developing coeliac disease.

机译：对脱酰胺化的麦醇溶蛋白肽的抗体反应在发展中的乳糜泻中显示出高特异性和针对组织转谷氨酰胺酶的平行抗体。

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摘要

Coeliac disease (CD) is an enteropathy induced in genetically susceptible individuals by gluten components, gliadin, hordein and secalin, polypeptides present in cereals such as wheat, barley and rye, respectively. Although the disease starts as intolerance to gliadins, antibodies to tissue transglutaminase (tTG) in the gut epithelium are characteristic of the disease. Whereas serum autoantibodies against tTG (tTGA) are highly specific for CD, antibodies to gliadin are less informative as they can also be detected in other enteropathies, and even in healthy individuals. However, it was shown recently that antibodies to certain gliadin peptides occur with high specificity in CD patient sera. We developed a solid phase lanthanide-based immunofluorometric assay for simultaneous detection of serum IgA and IgG antibodies to a synthetic peptide derived from gamma gliadin of wheat comprising amino acids 86-103. Three glutamine residues of this native 18-mer peptide were replaced by glutamic acids and the peptide was biotinylated. Sera from 87 individuals who had undergone duodenal biopsy and were diagnosed with CD and from 81 healthy individuals were analysed for the presence of both IgA and IgG anti-gliadin peptide antibodies. The performance of the peptide AGA assay was excellent, showing a specificity and sensitivity of 90% and 92% for IgA, and 98% and 75% for IgG, respectively. The corresponding values for conventional anti-gliadin antibody (AGA) enzyme-linked immunosorbent assay (ELISA) tests were 72% specificity and 87% sensitivity for IgA, and 64% specificity and 78% sensitivity for IgG. In a prospective study, almost all the tTGA-positive sera drawn from children who later developed CD were also positive for gliadin peptide antibodies.
机译：乳糜泻（CD）是遗传易感个体中的一种肠病，由麸质成分，麦醇溶蛋白，大麦醇溶蛋白和secalin分别存在于谷物（例如小麦，大麦和黑麦）中引起。尽管该疾病以对麦醇溶蛋白的不耐受开始，但肠道上皮组织抗谷氨酰胺转移酶（tTG）的抗体仍是该疾病的特征。尽管针对tTG（tTGA）的血清自身抗体对CD具有高度特异性，但针对麦醇溶蛋白的抗体的信息量较少，因为它们也可以在其他肠病甚至健康个体中检测到。但是，最近显示在CD患者血清中以高特异性出现针对某些麦醇溶蛋白肽的抗体。我们开发了一种基于镧系元素的固相免疫荧光测定法，可同时检测血清IgA和IgG抗体，该抗体来自小麦的γ-醇溶蛋白（包含氨基酸86-103）。该天然的18聚体肽的三个谷氨酰胺残基被谷氨酸替代，并且该肽被生物素化。分析来自87位经十二指肠活检并被诊断为CD的个体的血清以及来自81位健康个体的IgA和IgG抗麦醇溶蛋白抗体的存在。肽AGA分析的性能出色，对IgA的特异性和灵敏度分别为90％和92％，对IgG分别为98％和75％。传统抗麦醇溶蛋白抗体（AGA）酶联免疫吸附试验（ELISA）的相应值分别为：对IgA的特异性为72％和87％，对IgG的特异性为64％和78％。在一项前瞻性研究中，从后来发展为CD的儿童中抽取的几乎所有tTGA阳性血清也对麦醇溶蛋白肽抗体呈阳性。

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