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首页> 外文期刊>魚病研究 >Development of a PCR-RFLP Method for Differentiation of Perkinsus olseni and P. honshuensis in the Manila Clam Ruditapes philippinarum
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Development of a PCR-RFLP Method for Differentiation of Perkinsus olseni and P. honshuensis in the Manila Clam Ruditapes philippinarum

机译：PCR-RFLP方法在马尼拉蛤仔菲律宾蛤仔和白头翁的鉴别中的应用

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摘要

We developed a method using PCR-RFLP to differentiate Perkinsus olseni and P. honshuensis, the latter of which was recently discovered as a new species in Mie, Japan, in the Manila clam Ruditapes philippinarum. In normal gill samples spiked with cultured trophozoites of the parasites, the minimum infection levels that could be detected by this method were100 cells per10 mg sample for P. olseni and one cell for P. honshuensis. Using this method, we found that clams from the western Seto Inland Sea were infected with both species of the parasites. This is the first report of P. honshuensis from areas outside the type locality of this species.
机译：我们开发了一种使用PCR-RFLP区分白kin（Perkinsus olseni）和本氏疟原虫（P. honshuensis）的方法，后者最近在日本三重县的马尼拉蛤仔菲律宾蛤d（Rudetapes philippinarum）被发现为新物种。在掺有培养的滋养体滋养体的正常g样品中，用此方法可检测到的最低感染水平是：每10 mg的P. olseni样品有100个细胞，而本州的P. honshuensis则有一个细胞。使用这种方法，我们发现来自濑户内海西部的蛤lam感染了两种寄生虫。这是本州P. honsensis的首次报道。

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