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首页> 外文期刊>Biopreservation and biobanking >Xeno-Free Cryopreservation of Bone Marrow-Derived Multipotent Stromal Cells from Callithrix jacchus
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Xeno-Free Cryopreservation of Bone Marrow-Derived Multipotent Stromal Cells from Callithrix jacchus

机译：Callithrix jacchus骨髓衍生的多能干基质细胞的无异种冷冻保存

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In the previous decade, numerous biobanks were established and have created large markets for the storage of bioactive compounds, cells, and tissues for medical and diagnostic applications. For in vivo clinical and therapeutic purposes, it is critical to use well-defined and xeno-free components during cultivation, preservation, and transplantation of biological material. Safe and efficacious storage of bioactive molecules, cells, and tissues, without the addition of undefined medium components, minimizes risks of zoonotic disease transmission and is thus an essential and desirable prerequisite for biobanks. This gives rise to a need for well-characterized and serum-free freezing media for application in cryopreservation. For this purpose, cryobiological additives such as methylcellulose, poloxamer-188, and alpha-tocopherol, which have previously been shown to exhibit a cytoprotective activity, have been investigated for cryoprotection on stem cells. With this strategy, the application of fetal bovine serum(FBS) could be avoided and the concentration of toxic cryoprotective agents such as dimethyl sulfoxide (DMSO) could be reduced. Our results suggest that the viability, as well as the adipogenic and osteogenic differentiation capacity of the thawed bone marrow-derived multipotent stromal stem cells, could be maintained using a freezing medium without FBS consisting of methylcellulose, poloxamer, and alpha-tocopherol with only 2.5% DMSO (% v/v).
机译：在过去的十年中，建立了许多生物库，并为医疗和诊断应用的生物活性化合物，细胞和组织的存储创造了广阔的市场。为了体内临床和治疗目的，在生物材料的培养，保存和移植过程中使用定义明确且不含异种的成分至关重要。在不添加不确定的培养基成分的情况下，安全有效地存储生物活性分子，细胞和组织，可将人畜共患疾病的传播风险降到最低，因此是生物库必不可少的理想先决条件。这就需要用于冷冻保存的特性良好且无血清的冷冻培养基。为了这个目的，已经研究了冷冻生物学添加剂如甲基纤维素，泊洛沙姆-188和α-生育酚，它们先前已显示出具有细胞保护活性，已被研究用于对干细胞进行冷冻保护。通过这种策略，可以避免胎牛血清（FBS）的应用，并可以降低有毒的冷冻保护剂（如二甲基亚砜（DMSO））的浓度。我们的研究结果表明，使用不含甲基纤维素，泊洛沙姆和α-生育酚的FBS（仅含2.5）的冷冻培养基，可以维持融化的骨髓来源的多能干基质干细胞的活力以及成脂和成骨分化能力。 ％DMSO（％v / v）。

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