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首页> 外文期刊>日本家禽学会誌 >Effects of thyroid hormone on serum insulin-like growth factor binding proteins (IGFBPs) and IGFBP-2 mRNA expression in chickens.
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Effects of thyroid hormone on serum insulin-like growth factor binding proteins (IGFBPs) and IGFBP-2 mRNA expression in chickens.

机译：甲状腺激素对鸡血清胰岛素样生长因子结合蛋白（IGFBPs）和IGFBP-2 mRNA表达的影响。

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摘要

We examined serum IGF binding proteins (IGFBPs) and tissue (liver, kidney and brain) IGFBP-2 mRNA in 5-week-old cockerels with different thyroid status to elucidate a possible role of thyroid hormone on IGFBPs in the chicken. Assay for serum IGFBPsby Western ligand blot detected three IGFBPs having molecular masses of 30, 36 and 41 kDa. The 125I-IGF-I bindings to these IGFBPs were significantly decreased in propylthiouracil (PTU) induced hypothyroid cockerels, and restored to euthyroid control levels by thyroxine (T4) supplement. T4 supplement to euthyroid birds had no significant influence on 125I-IGF-I binding to IGFBPs. The expression of IGFBP-2 mRNA in the liver was significantly reduced in hypothyroidism and restored to normal level by T4 replacement, whereas T4, injected to intact birds, failed to affect hepatic IGFBP-2 mRNA expression. In contrast, the expression of IGFBP-2 mRNA in the kidney was increased in hypothyroid birds and decreased both in T4-treated intact and hypothyroid birds.In the brain, IGFBP-2 mRNA level was not influenced by the changes in thyroid status. IGFBP-2 mRNA was detected in all tissues examined, with varying degree. The expression was higher in the testis, gizzard, brain, eye and bursa of Fabricius, followed by kidney and digestive organs. In the liver, the expression was very low compared with other tissues.
机译：我们检查了具有不同甲状腺状态的5周龄公鸡的血清IGF结合蛋白（IGFBP）和组织（肝脏，肾脏和大脑）IGFBP-2 mRNA，以阐明甲状腺激素对鸡中IGFBP的可能作用。通过Western配体印迹法测定血清IGFBP，检测到三种分子量为30、36和41 kDa的IGFBP。在丙硫氧嘧啶（PTU）引起的甲状腺机能减退的公鸡中，与这些IGFBP的125I-IGF-I结合显着降低，并通过甲状腺素（T4）补充物恢复到正常的甲状腺控制水平。甲状旁腺鸟的T4补充剂对125I-IGF-I与IGFBP的结合没有显着影响。在甲状腺功能减退症中，肝脏中IGFBP-2 mRNA的表达显着降低，并通过T4替代而恢复至正常水平，而注射至完整禽类的T4则不会影响肝脏IGFBP-2 mRNA的表达。相比之下，甲状腺功能减退的鸟类的肾脏中IGFBP-2 mRNA的表达增加，而经T4处理的完整和甲状腺功能减退的鸟类的肾脏中IGFBP-2 mRNA的表达均降低。在检查的所有组织中均以不同程度检测到IGFBP-2 mRNA。在Fabricius的睾丸，g，大脑，眼睛和法氏囊中表达较高，其次是肾脏和消化器官。与其他组织相比，在肝脏中的表达非常低。

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