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首页> 外文期刊>日本家禽学会誌 >Temperature-dependent immobilization and restoration of fowl sperm motility caused by intracellular free calcium
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Temperature-dependent immobilization and restoration of fowl sperm motility caused by intracellular free calcium

机译：温度依赖性固定和恢复细胞内游离钙引起的家禽精子运动

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Fowl spermatozoa maintained vigorous movement at 30oC with or without Ca2+. In contrast, the motility was reversibly inhibited at 40oC without Ca2+, but could be immediately restored by the addition of Ca2+. However, the addition of verapamil, a specific Ca2+ channel blocker, before the addition of Ca2+, prevented full motility of spermatozoa at 40oC. Under these conditions, the intracellular free Ca2+ concentration was lower than that of the control (no addition of verapamil), as measured by a fluorescent Ca2+ indicator, fura-2. The preparations of spermatozoa without Ca2+ were incubated at 30oC, and then transferred and incubated at 40oC. After that, they were re-incubated at 30oC again. During the second incubation at 30oC, high motility was obtained, even in the presence of verapamil. The addition of Sr2+, which appears to stimulate the release of Ca2+ from the mitochondria, was also effective in stimulating motility at 40oC, and induced a concomitant increase in intracellular free Ca2+ concentration. The results suggest that intracellular free Ca2+ plays a pivotal role in regulating fowl sperm motility, and it appears that fowl sperm mitochondria have a significant role in controlling intracellular free Ca2+ concentrations. This mechanism,rather than Ca2+ fluxes through the plasma membrane, may be involved in the temperature-dependent immobilization and restoration of fowl sperm motility.
机译：有或没有Ca 2+的条件下，家禽精子在30°C都能保持剧烈运动。相反，在没有Ca2 +的情况下，运动性在40oC时被可逆地抑制，但是可以通过添加Ca2 +立即恢复。但是，在添加Ca2 +之前添加维拉帕米（一种特定的Ca2 +通道阻滞剂）会阻止40°C时精子的完全运动。在这些条件下，通过荧光Ca2 +指示剂fura-2测量，细胞内游离Ca2 +浓度低于对照（未添加维拉帕米）。将不含Ca2 +的精子制剂在30°C下孵育，然后转移并在40°C下孵育。之后，将它们再次在30oC下孵育。在30°C的第二次孵育过程中，即使存在维拉帕米，也能获得高运动性。 Sr2 +的添加似乎刺激了线粒体中Ca2 +的释放，在40oC时也能有效刺激运动，并引起细胞内游离Ca2 +浓度的同时增加。结果表明，细胞内游离Ca2 +在调节家禽精子活力中起着关键作用，并且看来家禽精子线粒体在控制细胞内游离Ca2 +浓度中具有重要作用。这种机制，而不是Ca2 +通过质膜的通量，可能与温度依赖的固定和家禽精子运动的恢复有关。

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