• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>日本兽医学杂誌 >Cloning and expression of mitochondrial capsule selenprotein gene in the golden hamster
【24h】

Cloning and expression of mitochondrial capsule selenprotein gene in the golden hamster

机译：金黄仓鼠线粒体胶囊硒蛋白基因的克隆与表达

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mitochondrial capsule selenoprotein (MCS) has been known as a structural protein of the mitochondrial sheath in spermatozoa. In the present study, a full-length cDNA encoding the MCS was first isolated from the testes of 10-week-old golden hamsters using a RACE (rapid amplification of cDNA ends) technique and its mRNA expression pattern was investigated from the hamster tissues by reverse transcription-polymerase chain reaction (AT-NCR) analysis. Hamster MCS cDNA was 820 bp long, including 24 bp of the 5'-untranslated region (UTR) and 243 bp of the 3'-UTR, and showed identity of 75.6% and 73.9% with mouse and rat MCS. According to the deduced amino acid (aa) sequence analysis, hamster MCS encoded a polypeptide of 184 aa, including a cysteine- and proline-rich domain which is the characteristic sequences of MCS, and contained 2 in-frame UGA codons for selenocysteine. Hamster MCS also shared aa identity of 64.4% with mouse MCS and contained an Arg-Lys-Ser-Thr-rich region in the N-terminus similar to the mitochondrial targeting signal. On the other hand, according to the AT-NCR analysis using the specific primers for hamster MCS, hamster MCS mRNA was expressed in various tissues as well as the testes. This finding indicates that MCS in hamster may have more than just a function of mitochondrial sheath formation of spermatozoa.
机译：线粒体胶囊硒蛋白（MCS）是精子中线粒体鞘的结构蛋白。在本研究中，首先使用RACE（cDNA末端的快速扩增）技术从10周龄的金黄仓鼠的睾丸中分离出编码MCS的全长cDNA，并通过仓鼠组织研究了其mRNA表达模式。逆转录聚合酶链反应（AT-NCR）分析。仓鼠MCS cDNA长820 bp，包括24 bp的5'-非翻译区（UTR）和243 bp的3'-UTR，与小鼠和大鼠MCS的同一性分别为75.6％和73.9％。根据推导的氨基酸（aa）序列分析，仓鼠MCS编码了一个184 aa的多肽，包括一个富含半胱氨酸和脯氨酸的结构域，是MCS的特征序列，并包含两个框内UGA密码子，用于半胱氨酸。仓鼠MCS还与小鼠MCS共享64.4％的氨基酸同一性，并且在N端包含类似于线粒体靶向信号的富含Arg-Lys-Ser-Thr的区域。另一方面，根据使用仓鼠MCS的特异性引物的AT-NCR分析，仓鼠MCS mRNA在各种组织以及睾丸中表达。该发现表明仓鼠中的MCS可能不仅具有精子的线粒体鞘形成功能。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号