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Microarrays for public health: genomic epidemiology of tuberculosis

机译：用于公共卫生的微阵列：结核病的基因组流行病学

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摘要

In response to a large local school-based outbreak of tuberculosis, we have been evaluating the utility of microarray bacterial genomic analysis in outbreak management. After initial comparison of the isolate from the index case with Mycobacterium tuberculosis H37Rv, it was possible to design robust PCRs directed towards strain-specific deletions. Rapid PCR analysis of isolates proved valuable in determining whether or not other isolates were compatible with the outbreak strain and further microarray studies revealed genetic markers that could be used to discriminate between locally circulating strains. We suggest that this approach forms the basis for developing rapid local genotyping schemes applicable to M. tuberculosis and that application to other pathogens warrants consideration.
机译：针对大规模的本地学校结核病暴发，我们一直在评估微阵列细菌基因组分析在暴发管理中的效用。在与结核分枝杆菌H37Rv对来自索引病例的分离株进行初步比较之后，有可能设计出针对菌株特异性缺失的稳健PCR。分离株的快速PCR分析被证明对确定其他分离株是否与暴发菌株兼容具有重要价值，进一步的微阵列研究揭示了可用于区分局部循环菌株的遗传标记。我们建议这种方法构成了发展适用于结核分枝杆菌的快速局部基因分型方案的基础，并且对其他病原体的应用值得考虑。

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