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A computational strategy for protein function assignment which addresses the multidomain problem

机译:蛋白质功能分配的计算策略,解决了多域问题

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摘要

A method for assigning functions to unknown sequences based on finding correlations between short signals and functional annotations in a protein database is presented. This approach is based on keyword (KW) and feature (FT) information stored in the SWISS-PROT database. The former refers to particular protein characteristics and the latter locates these characteristics at a specific sequence position. In this way, a certain keyword is only assigned to a sequence if sequence similarity is found in the position described by the FT field. Exhaustive tests performed over sequences with homologues (cluster set) and without homologues (singleton set) in the database show that assigning functions is much 'cleaner' when information about domains (FT field) is used, than when only the keywords are used.
机译:提出了一种基于在蛋白质数据库中发现短信号和功能注释之间的相关性,将功能分配给未知序列的方法。该方法基于存储在SWISS-PROT数据库中的关键字(KW)和功能(FT)信息。前者是指特定的蛋白质特征,而后者是指将这些特征定位在特定的序列位置。这样,只有在FT字段描述的位置中发现了序列相似性时,才将某个关键字分配给序列。对数据库中具有同源物(簇集)和没有同源物(单例集)的序列进行的详尽测试表明,与仅使用关键字相比,使用域信息(FT字段)时分配功能要“干净”得多。

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