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Microarray analysis of bacterial gene expression: towards the regulome

机译:细菌基因表达的微阵列分析:正向规律

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摘要

Microarray technology allows co-regulated genes to be identified. In order to identify genes that are controlled by specific regulators, gene expression can be compared in mutant and wild-type bacteria. However, there are a number of pitfalls with this approach; in particular, the regulator may not be active under the conditions in which the wild-type strain is cultured. Once co-regulated genes have been identified, protein-binding motifs can be identified. By combining these data with a map of promoters, or operons the operome), the regulatory networks in the cell (the regulome) can start to be built up.
机译:微阵列技术可以识别共同调控的基因。为了鉴定受特定调节剂控制的基因,可以在突变型细菌和野生型细菌中比较基因表达。但是,这种方法有很多陷阱。特别地,调节剂在培养野生型菌株的条件下可能没有活性。一旦确定了共同调控的基因,就可以确定蛋白结合基序。通过将这些数据与启动子或操纵子的操纵子图结合,可以开始建立细胞中的调节网络(正当基因组)。

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