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首页> 外文期刊>感染症学雑誌 >Usefulness of arbitrarily primed polymerase chain reaction (AP-PCR) method for DNA fingerprinting analysis of Mycobacterium tuberculosis (MTB); comparison between DNA restriction fragment-length polymorphism (RFLP) method and AP-PCR method
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Usefulness of arbitrarily primed polymerase chain reaction (AP-PCR) method for DNA fingerprinting analysis of Mycobacterium tuberculosis (MTB); comparison between DNA restriction fragment-length polymorphism (RFLP) method and AP-PCR method

机译：任意引发的聚合酶链反应（AP-PCR）方法在结核分枝杆菌（MTB）DNA指纹分析中的用途; DNA限制性片段长度多态性（RFLP）法与AP-PCR法的比较

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摘要

We have been analyzing cases suspected as outbreak of Mycobacterium tuberculosis in Tokyo using RFLP method. This time we analyzed 27 strains of MTB from 5 cases in two hospitals, a family, member of social activity and stuff of a corporation using both RFLP and AP-PCR methods. At 4 cases, over 80% of strains were same pattern in each cases with RFLP and AP-PCR and were identified as a patient to patients transmission of MTB. At one case, in a hospital, each strains were completely different patterns at both methods, which showed it was not a outbreak case. Results of RFLP and AP-PCR were completely same, which indicates AP-PCR is also useful and rapid method for epidemiological analysis of MTB infection as well as RFLP.
机译：我们已经使用RFLP方法分析了东京疑似结核分枝杆菌暴发的病例。这次我们使用RFLP和AP-PCR方法分析了两家医院，一家人，社会活动成员和一家公司的5例病例中的27株MTB。在4例中，使用RFLP和AP-PCR在每种情况下超过80％的菌株具有相同的模式，并被确定为MTB的患者。在一个案例中，在一家医院中，两种方法中每种菌株的模式完全不同，这表明这不是爆发病例。 RFLP和AP-PCR的结果完全相同，这表明AP-PCR也是对MTB感染和RFLP进行流行病学分析的有用且快速的方法。

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