...
首页> 外文期刊>感染症学雑誌 >Epidemiological analysis of Salmonella enteritidis isolates using pulsed-field gel electrophoresis and bacteriophage typing over the period of April 2000 to March 2003 in Gifu Prefecture
【24h】

Epidemiological analysis of Salmonella enteritidis isolates using pulsed-field gel electrophoresis and bacteriophage typing over the period of April 2000 to March 2003 in Gifu Prefecture

机译:2000年4月至2003年3月在岐阜县使用脉冲场凝胶电泳和噬菌体分型对肠炎沙门氏菌进行流行病学分析

获取原文
获取原文并翻译 | 示例
           

摘要

We examined a total of 151 Salmonella enterica serovar Enteritidis strains isolated in Gifu Prefecture during the period from April 2000 to March 2003 by using bacteriophage typing and pulsed-field gel electrophoresis (PFGE). Bacteriophage typing classified them into twelve phage types (PT) and RDNC (reacted but did not conform). The predominant phage type was PT47 (34.4%) followed by PT1 (21.9%), PT4 (16.6%) and RDNC (11.3%). XbaI- and BlnI-digested PFGE analyses identified 17 and 44 PFGE patterns, respectively, indicating that PFGE with BlnI had more discriminating power than that with XbaI. Combination of the phage types and PFGE types of BlnI could make 53 subtypes. Some isolates with the same phage type were subdivided into different PFGE types, but those with PT47 were not. PT47 isolates were derived from sporadic patients with gastroenteritis, food poisoning outbreaks and healthy carriers through the years. This suggests that PT47 is highly clonal and disseminates over our prefecture.
机译:我们使用噬菌体分型和脉冲场凝胶电泳(PFGE)技术检测了2000年4月至2003年3月期间在岐阜县分离的151株肠炎沙门氏菌肠炎沙门氏菌。噬菌体分型将它们分为十二种噬菌体类型(PT)和RDNC(已反应但不符合)。噬菌体的主要类型是PT47(34.4%),其次是PT1(21.9%),PT4(16.6%)和RDNC(11.3%)。 XbaI和BlnI消化的PFGE分析分别确定了17种和44种PFGE模式,表明使用BlnI的PFGE比使用XbaI的PFGE具有更大的辨别力。 BlnI的噬菌体类型和PFGE类型的组合可以产生53个亚型。某些具有相同噬菌体类型的分离株被细分为不同的PFGE类型,而具有PT47的分离株则没有。多年来,PT47分离株来自胃肠炎,食物中毒暴发和健康携带者的零星患者。这表明PT47是高度克隆的,并且在我们的县内传播。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号