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首页> 外文期刊>Comparative biochemistry and physiology, Part C. Pharmacology, toxicology and endocrinology: An international journal >Characterization of a cytosolic estrogen receptor and its up-regulation by 17 beta-estradiol and the xenoestrogen 4-tert-octylphenol in the liver of eelpout (Zoarces viviparus)
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Characterization of a cytosolic estrogen receptor and its up-regulation by 17 beta-estradiol and the xenoestrogen 4-tert-octylphenol in the liver of eelpout (Zoarces viviparus)

机译：乙醇中雌激素受体的特征及其17β-雌二醇和异雌激素4-叔辛基酚的上调（Zoarces viviparus）

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Estrogen binding activity was revealed in the cytosolic fraction of hepatic extracts from adult male and female eelpout (Zoarces viviparus). The binding moiety was characterized by a single class of high affinity binding sites (K-d = 0.59 +/- 0.05 nM in males and 1.06 +/- 0.10 nM in females). The affinity was significantly higher in males. Binding sites were satiable and binding capacity was significantly elevated in vitellogenic females (2.92 +/- 0.28 pmol/g) compared to males (1.67 +/- 0.11 pmol/g). The binding was specific to known estrogens but not to other tested steroids. The binding moiety was able to bind to DNA-cellulose and was extractable by high salt concentrations. A time-course study of estrogen binding activity in liver cytosol and of vitellogenin (Vtg) in plasma, after intraperitoneal (i.p.) injections of 17 beta-estradiol (E-2) in male eelpout, was carried out. It was shown that both are inducible by E-2. Estrogen binding activity was significantly elevated 48 h and Vtg 72 h after E-2 treatment. The binding moiety was hereafter designated as a cytosolic estrogen receptor (ER). The estrogenicity of 4-tert-octylphenol (OP) was evaluated by measuring ER and Vtg after i.p. treatment. OF-treatment increased both receptor levels and Vtg concentrations in male fish, indicating that OP acts as an estrogen in male eelpout. (C) 2000 Elsevier Science Inc. All rights reserved. [References: 62]
机译：在成年雄性和雌性幼体（Zoarces viviparus）的肝提取物的胞质部分中显示出雌激素结合活性。结合部分的特征是一类高亲和力结合位点（雄性K-d = 0.59 +/- 0.05 nM，雌性K-d = 1.06 +/- 0.10 nM）。男性的亲和力明显更高。与男性（1.67 +/- 0.11 pmol / g）相比，成卵的女性（2.92 +/- 0.28 pmol / g）的结合位点令人满意，结合能力显着提高。这种结合对已知的雌激素是特异性的，但对其他测试的类固醇不是特异性的。结合部分能够结合DNA纤维素，并且可以通过高盐浓度提取。腹膜内（i.p.）注射17雌二醇（E-2）于雄性大湿lp中后，进行了肝细胞溶胶中雌激素结合活性和血浆中卵黄蛋白原（Vtg）时程研究。结果表明，两者均可被E-2诱导。 E-2处理后48小时和72小时Vtg的雌激素结合活性显着提高。下文将结合部分称为胞质雌激素受体（ER）。 i.p.后通过测量ER和Vtg评估4-叔辛基苯酚（OP）的雌激素性。治疗。 OF处理增加了雄鱼的受体水平和Vtg浓度，这表明OP在雄性出没中充当雌激素。 （C）2000 Elsevier Science Inc.保留所有权利。 [参考：62]

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