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首页> 外文期刊>Comparative biochemistry and physiology, Part C. Pharmacology, toxicology and endocrinology: An international journal >Molecular cloning and characterization of three novel cytochrome P450 2D isoforms, CYP2D20, CYP2D27, and CYP2D28 in the Syrian hamster (Mesocricetus auratus)
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Molecular cloning and characterization of three novel cytochrome P450 2D isoforms, CYP2D20, CYP2D27, and CYP2D28 in the Syrian hamster (Mesocricetus auratus)

机译：叙利亚仓鼠（Mesocricetus auratus）中三种新型细胞色素P450 2D亚型CYP2D20，CYP2D27和CYP2D28的分子克隆和特征

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We cloned three novel cytochrome P450 (CYP) 2D cDNAs in the Syrian hamster (Mesocricetus auratus). Each clone contained an open reading frame of 1500 nucleotides encoding a protein of 500 amino acids. The deduced amino acid sequences of these had high identities with those of the other CYP2D members, therefore, the clones were assigned as CYP2D20, CYP2D27, and CYP2D28. Northern blot analysis showed that the CYP2D27 mRNA was expressed in liver, but not in kidney, small intestine, and brain, while the CYP2D20 and CYP2D28 mRNAs were not detected in these tissues examined. The expression of CYP2D27 mRNA in liver did not show sex difference and was not induced by either 3-methylcholanthrene or phenobarbital treatment. We characterized the enzyme activities of recombinant CYP2D27 expressed in COS-7 cells. The CYP2D27 protein had the bufuralol 1'-hydroxylase and debrisoquine 4-hydroxylase activities that are specific to the CYP2D subfamily. (C) 2000 Elsevier Science Inc. All rights reserved. [References: 29]
机译：我们在叙利亚仓鼠（Mesocricetus auratus）中克隆了三个新型细胞色素P450（CYP）2D cDNA。每个克隆包含一个1500个核苷酸的开放阅读框，编码500个氨基酸的蛋白质。这些推导的氨基酸序列与其他CYP2D成员具有高度同一性，因此，将这些克隆指定为CYP2D20，CYP2D27和CYP2D28。 Northern印迹分析显示CYP2D27 mRNA在肝脏中表达，但在肾脏，小肠和脑中不表达，而在这些检查的组织中未检测到CYP2D20和CYP2D28 mRNA。 CYP2D27 mRNA在肝脏中的表达未显示性别差异，也不被3-甲基胆碱或苯巴比妥治疗诱导。我们表征了在COS-7细胞中表达的重组CYP2D27的酶活性。 CYP2D27蛋白具有特定于CYP2D亚家族的bufuralol 1'-羟化酶和debrisoquine 4-羟化酶活性。 （C）2000 Elsevier Science Inc.保留所有权利。 [参考：29]

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