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首页> 外文期刊>Comparative biochemistry and physiology, Part C. Pharmacology, toxicology and endocrinology: An international journal >Molecular characterization and developmental expression of the aryl hydrocarbon receptor from the chick embryo
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Molecular characterization and developmental expression of the aryl hydrocarbon receptor from the chick embryo

机译：雏鸡胚中芳烃受体的分子表征和发育表达

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摘要

The aryl hydrocarbon receptor (AhR) was cloned from the chick embryo and its function and developmental expression characterized. Chicken AhR cDNA coded for 858 amino acid protein and 396 bp of 3' UTR. The basic helix-loop-helix domain exhibited 87-100% amino acid identity to avian, mammalian, and amphibian AhR, and 69-74% to piscine AhR. The PAS (Per-ARNT-Sim) region was slightly less well conserved with (a) 97% identity to other avian sequences, (b) 81-86% to amphibian and mammalian AhR, and (c) 64-69% with piscine AhR. The carboxy terminus diverged the most among species with less than 53% amino acid identity between chicken and any available mammalian and piscine AhR sequences. The chicken AhR mRNA and protein were 6.1 kb and 103 kDa, respectively. Chicken AhR dimerzed with human AhR nuclear translocator and bound the mammalian dioxin-response element in a ligand-dependent manner. AhR protein was detected in neural ganglia; smooth, cardiac, and skeletal muscle; and epithelium involved in epithelial-to-mesenchymal transformations, such as pituitary, gastrointestinal tract, limb apical-ectodermal ridge, and kidney collecting ducts. AhR mRNA was detected in all tissues expressing protein, except myocardium. Cytochrome P4501A4 mRNA was highly induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in a subset of tissues expressing AhR, including small intestine, liver, kidney, blood vessels, and outflow tract myocardium. In conclusion, the AhR sequence and function is highly conserved between birds and mammals, and although many tissues express AhR during chick embryo development, only a subset are responsive to TCDD induction of CYP1A4. (C) 2000 Elsevier Science Inc. All rights reserved. [References: 50]
机译：从雏鸡胚胎中克隆了芳烃受体（AhR），并对其功能和发育表达进行了表征。鸡AhR cDNA编码858个氨基酸蛋白和396 bp的3'UTR。基本的螺旋-环-螺旋结构域与禽类，哺乳动物和两栖类AhR表现出87-100％的氨基酸同一性，与鱼类AhR表现出69-74％的氨基酸同一性。 PAS（Per-ARNT-Sim）区域的保守性稍差，（a）与其他鸟类序列的同源性为97％，（b）与两栖动物和哺乳动物AhR的同源性为81-86％，以及（pi）的为64-69％ AhR。在鸡与任何可用的哺乳动物和鱼类AhR序列之间，氨基酸末端的羧基末端差异最大，物种之间的一致性不到53％。鸡的AhR mRNA和蛋白分别为6.1 kb和103 kDa。鸡AhR与人类AhR核转运蛋白二聚并以配体依赖性方式结合哺乳动物二恶英反应元件。在神经节中检测到AhR蛋白;平滑肌，心肌和骨骼肌;和上皮参与上皮到间质转化，例如垂体，胃肠道，四肢顶皮表皮和肾收集管。除心肌外，在所有表达蛋白质的组织中均检测到AhR mRNA。在一部分表达AhR的组织（包括小肠，肝，肾，血管和流出道心肌）中，2,3,7,8-四氯二苯并二恶英（TCDD）高度诱导了细胞色素P4501A4 mRNA的表达。总之，AhR序列和功能在鸟类和哺乳动物之间是高度保守的，尽管许多组织在雏鸡胚胎发育过程中表达AhR，但只有一部分对TCDD诱导的CYP1A4有反应。 （C）2000 Elsevier Science Inc.保留所有权利。 [参考：50]

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