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首页> 外文期刊>Comparative biochemistry and physiology, Part C. Pharmacology, toxicology and endocrinology: An international journal >Antibacterial properties and partial cDNA sequences of cecropin-like antibacterial peptides from the common cutworm, Spodoptera litura
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Antibacterial properties and partial cDNA sequences of cecropin-like antibacterial peptides from the common cutworm, Spodoptera litura

机译：斜纹夜蛾（Spodoptera litura）cecropin样抗菌肽的抗菌特性和部分cDNA序列

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The antibacterial properties and cDNA sequences of two types of antibacterial peptides from the haemolymph of immunized common cutworm, Spodoptera litura larvae, were determined. Since the primary structures of peptides deduced from cDNA sequences showed significant homologies to cecropins A and B: they were named as Spodoptera cecropins A and B. Spodoptera cecropins were broadly effective against Gram-positive and negative bacteria. They also retained antibacterial activities in all conditions tested (at pH 5.6-8.0 and in the presence of 50-150 mM NaCl) that was adapted to confirm the antibacterial properties of Spodoptera cecropins. These results indicate that the change of pH and the increase of salt concentration in the media do not influence the activities of Spodoptera cecropins. For the reverse transcription (RT)-polymerase chain reaction (PCR) to obtain the complete primary sequencer the primer designed according to the conserved region of the cecropin leader sequences was used, which was determined by the comparison of the cDNA sequences of known cecropins. The results from RT-PCR presented that the partial cDNAs of Spodoptera cecropins A and B encode 57 and 58 amino acids, including the sequences of mature peptides, respectively. In addition, Northern blot analysis with P-32-labeled PCR product coding for Spodoptera cecropin A revealed that Spodoptera cecropin genes are expressed in immunized fat body, but not in normal fat body, (C) 2000 Elsevier Science Inc. All rights reserved. [References: 34]
机译：确定了免疫的普通角虫斜纹夜蛾幼虫的血淋巴中的两种抗菌肽的抗菌特性和cDNA序列。由于从cDNA序列推导的肽的一级结构与天蚕素A和B表现出显着的同源性：它们被命名为Spodoptera cecropins A和B。斜纹夜蛾cecropins对革兰氏阳性和阴性细菌具有广泛的作用。他们还保留了在所有测试条件下（pH 5.6-8.0且存在50-150 mM NaCl的条件下）的抗菌活性，这些条件适用于确认斜纹夜蛾的抗菌特性。这些结果表明，培养基中pH的变化和盐浓度的增加不会影响斜纹夜蛾的活动。为了获得完整的一级测序仪，进行逆转录（RT）-聚合酶链反应（PCR），使用了根据天蚕素前导序列保守区域设计的引物，该引物是通过比较已知天蚕素的cDNA序列确定的。 RT-PCR的结果表明，斜纹夜蛾Acrocropins A和B的部分cDNA分别编码57和58个氨基酸，包括成熟肽的序列。另外，用编码斜纹夜蛾cecropin A的P-32标记的PCR产物进行Northern印迹分析表明，斜纹夜蛾cecropin基因在免疫的脂肪体中表达，而在正常脂肪体中不表达，（C）2000 Elsevier Science Inc.保留所有权利。 [参考：34]

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