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首页> 外文期刊>感染症学雑誌 >The usefulness of human lung embryonal fibroblast cells (MRC-5) for isolation of enteroviruses and adenoviruses
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The usefulness of human lung embryonal fibroblast cells (MRC-5) for isolation of enteroviruses and adenoviruses

机译：人肺胚胎成纤维细胞（MRC-5）用于分离肠病毒和腺病毒的有用性

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Although various cell lines have been used for virus isolation, few study of virus isolation using MRC-5 cell, a human embryonic lung fibroblasts, have been reported in Japan. MRC-5 and other cell lines (Caco-2, Vero, RD-18s, LLC-MK2, HeLa, MDCK, FL, B95a and HMV-II), and suckling mouse were compared for isolation of viruses from clinical specimens. A total of 3,284 specimens, collected from clinics and hospitals in Saitama Prefecture between July 1997 and August 2001, were inoculated in these cells. A total of 1,252 viral strains were isolated and 1,190 viral strains of these were identified. MRC-5 detected 209 of specimens positive for various viruses. As for adenovirus, a total of 132 viral strains were isolated using cell lines described above, and 100 of 132 viral strains were isolated in MRC-5. MRC-5 showed the highest sensitivity for isolation of adenovirus 3 and 7 (79.1% and 100%) of all other cells. The sensitivity in isolation of these viruses in HeLa was 58.1% and 50.0%, respectively. It showed that MRC-5 is able to isolate enterovirus, especially coxsackie virus A16 and enterovirus 71 with a high sensitivity (85.7% and 73.7%). RD-18s detected 35.7% and 26.3% of coxsackie virus A16 and enterovirus 71 isolates, LLC-MK2 detected 60.7% and 47.4%, and Vero detected 48.6% and 52.6%, respectively. Coxsackie virus B group was not isolated, except for a few coxsackie virus B 5 strains. Enteroviruses except coxsackie virus A16 and enterovirus 71 were isolated more frequently in Caco-2 and RD-18s. Seven hundred thirteen strains of influenza viruses were isolated in MDCK and Caco-2, but none was isolated in MRC-5. It was probably due to the maintenance medium without trypsin. The isolation rate of herpes simplex virus in Vero was 88.9% and MRC-5 showed 77.8%, it was high secondary to Vero by MRC-5. However, the CPE was detected in a few days in MRC-5, it was earlier than in Vero. The MRC-5 is possible to be maintained without changing the maintenance medium and passaged for 2 weeks, and clear CPE was observed. On the other hand, the disadvantages in using the MRC-5 were that the passage was limited and that the split ratio was only 1:2. However, the MRC-5 was used successfully for virus isolation, especially coxsackie virus A16, enterovirus 71 and adenoviruses, from clinical specimens.
机译：尽管各种细胞系已经用于病毒分离，但是在日本，关于使用人胚胎肺成纤维细胞MRC-5细胞进行病毒分离的研究很少。比较了MRC-5和其他细胞系（Caco-2，Vero，RD-18s，LLC-MK2，HeLa，MDCK，FL，B95a和HMV-II）和乳鼠从临床标本中分离病毒的能力。在这些细胞中接种了1997年7月至2001年8月间从ita玉县诊所和医院收集的3,284个标本。总共分离出了1,252株病毒株，其中鉴定出1,190株病毒株。 MRC-5检测到209种对各种病毒呈阳性的标本。对于腺病毒，使用上述细胞系分离出总共132个病毒株，并且在MRC-5中分离了132个病毒株中的100个。 MRC-5对所有其他细胞中分离出腺病毒3和7的敏感性最高（分别为79.1％和100％）。在HeLa中分离这些病毒的敏感性分别为58.1％和50.0％。结果表明，MRC-5能够高灵敏度（85.7％和73.7％）分离肠病毒，尤其是柯萨奇病毒A16和肠病毒71。 RD-18s检测出柯萨奇病毒A16和肠道病毒71株分离株的35.7％和26.3％，LLC-MK2分别检测到60.7％和47.4％，Vero分别检测到48.6％和52.6％。除少数柯萨奇病毒B 5株外，未分离出柯萨奇病毒B组。除柯萨奇病毒A16和肠道病毒71外，肠道病毒在Caco-2和RD-18s中的分离频率更高。在MDCK和Caco-2中分离出了713种流感病毒，而在MRC-5中没有分离出。这可能是由于没有胰蛋白酶的维持培养基。 Vero中单纯疱疹病毒的分离率为88.9％，MRC-5的分离率为77.8％，MRC-5对Vero的继发率较高。但是，几天后在MRC-5中检测到了CPE，它比在Vero中更早。无需更换维护介质即可维护MRC-5，并通过2周，观察到透明的CPE。另一方面，使用MRC-5的缺点是通道受到限制，分流比仅为1：2。但是，MRC-5已成功用于从临床标本中分离病毒，尤其是柯萨奇病毒A16，肠病毒71和腺病毒。

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