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Targeting Cryopreservation-Induced Cell Death: A Review

机译：针对冷冻保存诱导的细胞死亡：审查。

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摘要

Despite marked developments in the field of cryopreservation of cells and tissues for research and therapeutic applications, post-thaw cell death remains a significant drawback faced by cryobiologists. Post cryopreservation apoptosis and necrosis are normally observed within 6 to 24 h after post-thaw culture. As a result, massive loss of cell viability and cellular function occur due to cryopreservation. However, in this new generation of cryopreservation science, scientists in this field are focusing on incorporation of apoptosis and necrosis inhibitors (zVAD-fmk, p38 MAPK inhibitor, ROCK inhibitor, etc.) to cryopreservation and post-thaw culture media. These inhibitors target and inhibit various proteins such as caspases, proteases, and kinases, involved in the cell death cascade, resulting in reduced intensity of apoptosis and necrosis in the cryopreserved cells and tissues, increased cell viability, and maintenance of cellular function; thus improved overall cryopreservation efficiency is achieved. The present article provides an overview of various cell death pathways, molecules mediating cryopreservation-induced apoptosis and the potential of certain molecules in targeting cryopreservation- induced delayed-onset cell death.
机译：尽管在用于研究和治疗应用的细胞和组织的冷冻保存领域中有显着的发展，但是解冻后的细胞死亡仍然是冷冻生物学家面临的重大缺陷。通常在融化后培养后6至24小时内观察到冷冻后的凋亡和坏死。结果，由于冷冻保存，导致细胞活力和细胞功能的大量损失。然而，在新一代的冷冻保存科学中，该领域的科学家致力于将细胞凋亡和坏死抑制剂（zVAD-fmk，p38 MAPK抑制剂，ROCK抑制剂等）掺入冷冻保存和融化后的培养基中。这些抑制剂靶向并抑制参与细胞死亡级联反应的各种蛋白质，例如胱天蛋白酶，蛋白酶和激酶，从而导致低温保存的细胞和组织的凋亡强度和坏死强度降低，细胞活力增强以及细胞功能维持;因此，提高了总体冷冻保存效率。本文概述了各种细胞死亡途径，介导冷冻保存诱导的细胞凋亡的分子以及某些分子靶向冷冻保存诱导的延迟发作的细胞死亡的潜力。

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