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首页> 外文期刊>Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies >Ethanolic cofermentation with glucose and xylose by the recombinant industrial strain Saccharomyces cerevisiae NAN-127 and the effect of furfural on xylitol production
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Ethanolic cofermentation with glucose and xylose by the recombinant industrial strain Saccharomyces cerevisiae NAN-127 and the effect of furfural on xylitol production

机译：重组酿酒酵母NAN-127与葡萄糖和木糖的乙醇乙醇发酵以及糠醛对木糖醇生产的影响

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摘要

Saccharomyces cerevisiae strain NAN-127 (2n, prototroph), which contains the xylose reductase-xylitol dehydrogenase (XR-XDH) xylose metabolic pathway was used for the cofermentation of glucose and xylose. Oxygen supply was the most important factor for xylose fermentation and pH 4.5 and a ventilation rate of 0.04 vvm were optimal. The xylose utilization ratio reached 0.655 at an initial xylose concentration of 50 g L-1 and was 0.9 at an initial concentration of 20 g L-1. Addition of furfural at late logarithmic phase as electron acceptor to a final concentration of 3.0 g L-1 decreased the xylitol yield by 17% under micro-aeration conditions without inhibiting cell growth, but also without an increase in ethanol yield. The results are important to the application of strain NAN-127 in the lignocellulosic ethanol process.
机译：包含木糖还原酶-木糖醇脱氢酶（XR-XDH）木糖代谢途径的酿酒酵母菌株NAN-127（2n，原养型）用于葡萄糖和木糖的发酵。氧气供应是木糖发酵的最重要因素，pH为4.5，通风速率为0.04 vvm最佳。在初始木糖浓度为50 g L-1时，木糖利用率为0.655，在初始木糖浓度为20 g L-1时，木糖利用率为0.9。在微对流条件下，在对数后期加入糠醛作为电子受体，使其终浓度为3.0 g L-1，木糖醇收率降低了17％，而没有抑制细胞生长，但乙醇收率也没有增加。结果对于菌株NAN-127在木质纤维素乙醇工艺中的应用很重要。

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