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首页> 外文期刊>Comparative Medicine >Susceptibility of irradiated B6D2F(1)/J mice to Klebsiella pneumoniae administered intratracheally: A pulmonary infection model in an immunocompromised host
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Susceptibility of irradiated B6D2F(1)/J mice to Klebsiella pneumoniae administered intratracheally: A pulmonary infection model in an immunocompromised host

机译：经气管内给药的B6D2F（1）/ J辐射小鼠对肺炎克雷伯菌的敏感性：免疫受损宿主中的肺部感染模型

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Bacteria such as Klebsiella pneumoniae can invade and colonize an immunocompromised host and complicate clinical recovery. In the study reported here, an experimental model of induced pneumonia was developed in Co-60 gamma-photon-irradiated mice for the purpose of evaluating efficacy of therapeutic agents. The model was characterized by use of probit analysis of bacterial dose, and microbiologic, and histopathologic results. Bacterial colony-forming-unit (CFU) values producing 50% mortality within 30 days (LD (50/30)) and their 95% confidence intervals were 4.0 x 10(4) [1.7 x 10(4) - 8.9 x 10(4)] for 0-Gray (Gy)-irradiated mice, 1.9 x 10(4) [7.0 x 10(3) - 4.8 x 10(4)] for 5-Gy-irradiated mice, and 1.0 x 10(3) [2.8 x 10(2) - 3.3 x 10(3)] for 7-Gy-irradiated mice. Probit regression line fits calculated by use of an iterative, weighted least-squares fit, were used to assess a dose-modifying factor (DMF). The DMFs for mortality, compared with that for the 0-Gy dose, with their 95% confidence intervals, were 2.2 [0.63 - 7.7] for the 5-Gy and 38.9 [9.6 - 165.0] for 7-Gy doses. The 5-Gy probit line did not significantly differ (P = 0.21) from the 0-Gy probit line (dose ratios did not significantly differ from 1), whereas the 7-Gy probit line differed significantly from the 0-Gy probit line (P < 0.001). These results demonstrate that 7-Gy Co-60 gamma-photon radiation in combination with intratracheal K pneumoniae challenge induces a valid pulmonary infection model in immunocompromised female B6D2F(1)/J mice.
机译：诸如肺炎克雷伯菌的细菌可以侵入并定殖于免疫受损的宿主，并使临床恢复复杂化。在此处报道的研究中，为了评估治疗剂的功效，在Co-60γ光子辐照的小鼠中建立了诱发性肺炎的实验模型。该模型的特征在于使用了细菌剂量，微生物学和组织病理学结果的概率分析。细菌菌落形成单位（CFU）值在30天内产生50％的死亡率（LD（50/30）），其95％置信区间为4.0 x 10（4）[1.7 x 10（4）-8.9 x 10（ 4）]（针对0-Gray（Gy）照射的小鼠，1.9 x 10（4）[7.0 x 10（3）-4.8 x 10（4）]，针对5-Gy照射的小鼠，以及1.0 x 10（3）对于7-Gy照射的小鼠，[2.8 x 10（2）-3.3 x 10（3）]。通过使用迭代加权最小二乘拟合计算出的Probit回归线拟合，用于评估剂量调整因子（DMF）。与0-Gy剂量相比，其DMF的死亡率为95％置信区间，其中5-Gy的DMF值为2.2 [0.63-7.7]，而7-Gy的剂量DMFs为38.9 [9.6-165.0]。 5-Gy概率曲线与0-Gy概率曲线无显着差异（P = 0.21）（剂量比与1差异不显着），而7-Gy概率曲线与0-Gy概率曲线显着不同（P = 0.21）。 P <0.001）。这些结果表明，7-Gy Co-60γ光子辐射与气管内肺炎克雷伯氏菌攻击相结合，可在免疫受损的雌性B6D2F（1）/ J小鼠中诱导有效的肺部感染模型。

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