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首页> 外文期刊>Comparative Medicine >A Mouse Surgical Model for Metastatic Ovarian Granulosa Cell Tumor.
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A Mouse Surgical Model for Metastatic Ovarian Granulosa Cell Tumor.

机译：转移性卵巢颗粒细胞瘤的小鼠手术模型。

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摘要

We recently described a genetically engineered mouse model that develops ovarian granulosa cell tumors (GCTs) that mimic many aspects of the advanced human disease, including distant dissemination. However, because the primary tumors killed their hosts before metastases were able to form, the use of these mice to study metastatic disease required the development of a simple, reliable, and humane surgical protocol for the excision of large GCTs from debilitated mice. Here we describe a protocol involving multimodal anesthesia, tumor removal through ventral midline celiotomy and perioperative fluid therapy, and analgesia that led to the postoperative survival of more than 90% of mice, despite the removal of tumors representing as much as 10% of the animal's body weight. Intraabdominal recurrence of the GCT did not occur in surviving animals, but most developed pulmonary or adrenal metastases (or both) by 12 wk after surgery. We propose that this mouse model of metastatic GCT will serve as a useful preclinical model for the development of novel treatment modalities and diagnostic techniques. Furthermore, our results delineate anesthetic and surgical principles for the removal of large abdominal tumors from mice that will be applicable to other models of human cancers.
机译：我们最近描述了一种基因工程小鼠模型，该模型可发展出卵巢颗粒细胞瘤（GCT），该颗粒可模仿人类晚期疾病的许多方面，包括远距离传播。但是，由于原发性肿瘤在转移形成之前就已经杀死了它们的宿主，因此，使用这些小鼠研究转移性疾病需要开发一种简单，可靠且人道的外科手术方案，以从衰弱的小鼠中切除大的GCT。在这里，我们描述了一种涉及多模式麻醉，通过腹侧中线开腹术和围手术期液体疗法清除肿瘤以及镇痛的方案，尽管尽管切除的肿瘤占动物的10％，但镇痛仍可导致超过90％的小鼠术后存活。体重。幸存的动物没有发生GCT的腹腔内复发，但是在手术后12周内，大多数发生了肺或肾上腺转移（或两者都有）。我们建议这种转移性GCT的小鼠模型将作为开发新型治疗方式和诊断技术的有用的临床前模型。此外，我们的研究结果描述了从小鼠中切除大腹部肿瘤的麻醉和手术原理，这些原理将适用于其他人类癌症模型。

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