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首页> 外文期刊>Comparative Medicine >Cloning and Comparison of Factor X from Rhesus Monkey (Macaca mulatta).
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Cloning and Comparison of Factor X from Rhesus Monkey (Macaca mulatta).

机译：恒河猴（Macaca mulatta）X因子的克隆与比较。

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摘要

The reliability of the rhesus monkey as an important experimental animal depends on its genetic concordance with human. During our assessment of the rhesus monkey as a preclinical model for coagulation-related research, we cloned the full-length cDNA of rhesus monkey factor X (FX) and compared its genetic characteristics and coagulation activity with those of human FX. The full-length cDNA of rhesus monkey FX was 1683 bp in length, corresponding to 487 coding amino acids and sharing 94.71% nucleotide identity and 93.65% amino acid identity with human FX. When FX sequences from different animals were compared with that of human FX, rhesus monkey and baboon FX showed similar degrees of homology to human FX, which were less than that between human and chimpanzee FX sequences but remarkably higher than those of another 2 monkey species, bovine, pig, and rodents. Comparison of functional sites between human and rhesus monkey FX revealed high similarities between their amino acids sequences and 3-dimensional structures. The average coagulation activity of FX from 24 rhesus monkeys was in the normal range of that of healthy humans. The rhesus monkey therefore may be a suitable animal model for research addressing coagulation factor X.
机译：恒河猴作为重要实验动物的可靠性取决于其与人类的遗传一致性。在我们将猕猴作为与凝血相关的研究的临床前模型进行评估的过程中，我们克隆了猕猴X因子（FX）的全长cDNA，并将其遗传特性和凝血活性与人FX进行了比较。恒河猴FX的全长cDNA长度为1683 bp，对应487个编码氨基酸，与人FX具有94.71％的核苷酸同一性和93.65％的氨基酸同一性。当将不同动物的FX序列与人FX的序列进行比较时，恒河猴和狒狒FX与人FX的相似程度相似，虽然比人和黑猩猩FX序列之间的相似度要低，但要比另外两种猴子物种高得多牛，猪和啮齿动物。人和恒河猴FX之间的功能位点比较表明，它们的氨基酸序列和3维结构之间具有高度相似性。 24只恒河猴的FX的平均凝血活性在健康人的正常范围内。因此，恒河猴可能是研究凝血因子X的合适动物模型。

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