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首页> 外文期刊>Comparative Medicine >Histopathologic Findings and Establishment of Novel Tumor Lines from Spontaneous Tumors in FVB/N Mice.
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Histopathologic Findings and Establishment of Novel Tumor Lines from Spontaneous Tumors in FVB/N Mice.

机译：FVB / N小鼠自发性肿瘤的组织病理学发现和新型肿瘤细胞系的建立。

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The inbred FVB mouse strain is used extensively in cancer research. Transgenic mice with an FVB/N background in which the expression of green fluorescent protein is under the control of various promoters have been used widely for the last decade. However, little is known about the incidence and characteristics of spontaneous tumors in these mice. In addition, only a few tumor lines have been established for use in this particular mouse strain. Our aim was to initiate a database of spontaneous tumors in our retired FVB/N breeders, analyze the histopathologic characteristics of these tumors, and establish novel tumor lines in vivo and in vitro. A total of 234 (40 male, 194 female) breeder mice were observed during their natural lifespans. The incidence of spontaneous tumors was 45.0% in male mice and 52.8% in female mice. All tumors in male mice were lung alveolar-bronchiolar (AB) neoplasms, except for 1 testis interstitial cell tumor. In female mice, histopathologic examination revealed 48 lung AB tumors, 27 mammary gland tumors, 13 ovarian tumors, and 14 other tumors. Several of these spontaneous tumors have been transplanted into FVB/N mice. One mammary adenocarcinoma (MCaP0008) and 1 lung AB carcinoma (LAP0297) were successfully transplanted subcutaneously and passaged serially in vivo. Subsequently, we established cell lines from both tumors, which were maintained in monolayer in vitro. Both of the grafted tumors and cell lines are tumorigenic in VEGF(P)-GFP/FVB and Tie2(P)-GFP/FVB mice. Establishment of these novel tumor lines will benefit both in vivo and in vitro studies on the pathophysiology of cancer in this relatively new but widely used mouse strain.
机译：自交系FVB小鼠品系广泛用于癌症研究。在过去的十年中，具有FVB / N背景的转基因小鼠被广泛使用，在FVB / N背景下绿色荧光蛋白的表达处于各种启动子的控制之下。但是，关于这些小鼠中自发性肿瘤的发病率和特征知之甚少。另外，仅建立了一些用于该特定小鼠品系的肿瘤系。我们的目标是在我们退休的FVB / N育种家中建立自发性肿瘤数据库，分析这些肿瘤的组织病理学特征，并在体内和体外建立新的肿瘤系。在其自然寿命中共观察到234只（40只雄性，194只雌性）种鼠。自发性肿瘤的发生率在雄性小鼠中为45.0％，在雌性小鼠中为52.8％。除1个睾丸间质细胞肿瘤外，雄性小鼠的所有肿瘤均为肺泡支气管（AB）肿瘤。在雌性小鼠中，组织病理学检查发现有48个肺部AB肿瘤，27个乳腺肿瘤，13个卵巢肿瘤和14个其他肿瘤。这些自发性肿瘤中的几种已被移植到FVB / N小鼠中。成功将1例乳腺腺癌（MCaP0008）和1例肺AB癌（LAP0297）皮下移植并在体内连续传代。随后，我们从两种肿瘤中建立了细胞系，并在体外单层维持。在VEGF（P）-GFP / FVB和Tie2（P）-GFP / FVB小鼠中，移植的肿瘤和细胞系均具有致瘤性。这些新型肿瘤系的建立将有益于在这种相对新的但被广泛使用的小鼠品系中对癌症的病理生理学的体内和体外研究。

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