• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Comparative Medicine >A Rat Model of Diabetic Wound Infection for the Evaluation of Topical Antimicrobial Therapies
【24h】

A Rat Model of Diabetic Wound Infection for the Evaluation of Topical Antimicrobial Therapies

机译：糖尿病伤口感染的大鼠模型用于局部抗菌治疗的评估

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Diabetes mellitus is an epidemic multisystemic chronic disease that frequently is complicated by complex wound infections. Innovative topical antimicrobial therapy agents are potentially useful for multimodal treatment of these infections. However, an appropriately standardized in vivo model is currently not available to facilitate the screening of these emerging products and their effect on wound healing. To develop such a model, we analyzed, tested, and modified published models of wound healing. We optimized various aspects of the model, including animal species, diabetes induction method, hair removal technique, splint and dressing methods, the control of unintentional bacterial infection, sampling methods for the evaluation of bacterial burden, and aspects of the microscopic and macroscopic assessment of wound healing, all while taking into consideration animal welfare and the '3Rs' principle. We thus developed a new wound infection model in rats that is optimized for testing topical antimicrobial therapy agents. This model accurately reproduces the pathophysiology of infected diabetic wound healing and includes the current standard treatment (that is, debridement). The numerous benefits of this model include the ready availability of necessary materials, simple techniques, high reproducibility, and practicality for experiments with large sample sizes. Furthermore, given its similarities to infected-wound healing and treatment in humans, our new model can serve as a valid alternative for applied research.
机译：糖尿病是一种流行性多系统慢性疾病，常常因复杂的伤口感染而变得复杂。创新的局部抗菌治疗剂可能可用于这些感染的多式联运治疗。但是，目前尚无适当标准化的体内模型来促进这些新兴产品的筛选及其对伤口愈合的影响。为了开发这样的模型，我们分析，测试和修改了已公布的伤口愈合模型。我们对模型的各个方面进行了优化，包括动物种类，糖尿病诱导方法，脱毛技术，夹板和敷料方法，无意细菌感染的控制，细菌负荷评估的采样方法以及微生物的微观和宏观评估伤口愈合，同时要考虑动物福利和“ 3R”原则。因此，我们在大鼠中开发了一种新的伤口感染模型，该模型已优化用于测试局部抗菌治疗剂。该模型可准确再现感染的糖尿病伤口愈合的病理生理，并包括当前的标准治疗方法（即清创术）。该模型的众多优点包括：随时可用的必要材料，简单的技术，高可重复性以及大样本量实验的实用性。此外，鉴于其与人类感染伤口的愈合和治疗相似，我们的新模型可以作为应用研究的有效替代方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号