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首页> 外文期刊>Comparative Medicine >Expression of Maspin in the Early Pregnant Mouse Endometrium and Its Role during Embryonic Implantation.
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Expression of Maspin in the Early Pregnant Mouse Endometrium and Its Role during Embryonic Implantation.

机译：Maspin在早孕小鼠子宫内膜中的表达及其在胚胎着床过程中的作用。

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Maspin (serpin B5), a tumor-suppressing member of the serine protease inhibitor family, participates in cell migration, adhesion, invasion, and apoptosis. These processes are also critical for embryo implantation, but the role of maspin in embryo implantation remains poorly understood. The aim of the present study was to investigate the spatiotemporal expression of maspin in early pregnant mouse endometrium and its role in embryo implantation. Real-time quantitative PCR analysis, immunohistochemistry, and Western blotting were used to detect mRNA and protein expression of maspin in the endometria of nonpregnant and early pregnant (days 0 to 7) mice. On day 3 of pregnancy, mice in the treated group (n = 20) were injected in the left uterine horn with antimaspin polyclonal antibody and in the right horn with purified rabbit IgG; control mice (n = 20) were injected only with purified rabbit IgG in the right uterine horn. Implanted embryos were counted on pregnant day 8. The mRNA and protein expressions of maspin were higher in the endometria of pregnant mice than nonpregnant mice; these levels gradually increased from day 1 of pregnancy, peaked on day 5, and then decreased on days 6 and 7. The mice treated with antimaspin polyclonal antibody group had far fewer implanted embryos than did the control group. Taken together, these results suggest that maspin, a tumor suppressor, may play an important role in embryo implantation.
机译：Maspin（serpin B5）是丝氨酸蛋白酶抑制剂家族的一种肿瘤抑制成员，参与细胞迁移，粘附，侵袭和凋亡。这些过程对于胚胎植入也很关键，但是人们对马斯平在胚胎植入中的作用还知之甚少。本研究的目的是研究maspin在早期妊娠小鼠子宫内膜中的时空表达及其在胚胎着床中的作用。实时定量PCR分析，免疫组化和Western印迹被用于检测未怀孕和早孕（第0至7天）小鼠子宫​​内膜中maspin的mRNA和蛋白表达。在怀孕的第3天，将治疗组（n = 20）的小鼠的左子宫角注射抗maspin多克隆抗体，并在右角注射纯化的兔IgG。对照组小鼠（n = 20）仅在右子宫角注射了纯化的兔IgG。在怀孕的第8天对植入的胚胎进行计数。maspin的mRNA和蛋白表达在怀孕小鼠的子宫内膜中高于未怀孕的小鼠。这些水平从怀孕第1天开始逐渐增加，在第5天达到峰值，然后在第6天和第7天下降。用抗Maspin多克隆抗体治疗的小鼠植入的胚胎比对照组少得多。综上所述，这些结果表明，抑癌物质maspin可能在胚胎植入中起重要作用。

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