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首页> 外文期刊>Molecular human reproduction. >Expression of calcium binding protein D-9k messenger RNA in the mouse uterine endometrium during implantation.
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Expression of calcium binding protein D-9k messenger RNA in the mouse uterine endometrium during implantation.

机译：钙结合蛋白D-9k信使RNA在小鼠子宫内膜的植入过程中的表达。

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摘要

To investigate the molecular mechanisms of implantation, we constructed a cDNA library of mouse uteri enriched with pregnancy-induced genes by subtractive hybridization and polymerase chain reaction (PCR). One of the isolated clones was the cDNA for the calcium binding protein D-9k (Cabp9k), which is considered to regulate intracytoplasmic concentration and transport of free calcium ions. Northern blot and in-situ hybridization analyses demonstrated that the Cabp9k mRNA was expressed in the endometrial epithelia, both luminal and glandular, in the uterus at the time of implantation. On pregnancy day 5 it was detected in the luminal, but not in the glandular, epithelia. In the oophorectomized adult mice, progesterone enhanced Cabp9k mRNA expression in the uterus, whereas oestrogen did not. Consistent with this, a nucleotide change was identified in the first intron of mouse Cabp9k gene corresponding to the oestrogen responsive element in the rat Cabp9k gene. Transfer of embryos into the uterine cavity of pseudopregnant mice reduced the expression of Cabp9k mRNA in the glandular epithelium, suggesting that Cabp9k mRNA expression is also regulated by embryonal signal(s). These findings demonstrated that Cabp9k mRNA is expressed in the endometrial epithelia during the implantation period under the control of progesterone and the presence of embryo, and suggest that CaBP9k plays a role in implantation by regulating the local calcium concentrations.
机译：为了研究植入的分子机制，我们通过消减杂交和聚合酶链反应（PCR）构建了小鼠子宫的cDNA库，该库富含妊娠诱导的基因。分离出的克隆之一是钙结合蛋白D-9k（Cabp9k）的cDNA，该蛋白被认为调节细胞质浓度和游离钙离子的转运。 Northern印迹和原位杂交分析表明，Cabp9k mRNA在植入时在子宫内膜和腺体的子宫内膜上皮中表达。在怀孕的第5天，在腔中检测到了，但在腺上皮中未检测到。在经去卵巢切除的成年小鼠中，孕酮增强子宫中的Cabp9k mRNA表达，而雌激素则不。与此相一致，在小鼠Cabp9k基因的第一个内含子中发现了与大鼠Cabp9k基因中的雌激素响应元件相对应的核苷酸变化。将胚胎转移到假孕小鼠的子宫腔中会降低腺上皮中Cabp9k mRNA的表达，这表明Cabp9k mRNA的表达也受胚胎信号的调节。这些发现表明，Capp9k mRNA在孕酮控制和胚胎存在的情况下在子宫内膜上皮中表达，并且表明CaBP9k通过调节局部钙浓度在子宫内膜中起作用。

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