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首页> 外文期刊>Comparative Medicine >Adaptation of Plasmodium vivax to growth in owl monkeys (Aotus nancymai)
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Adaptation of Plasmodium vivax to growth in owl monkeys (Aotus nancymai)

机译：间日疟原虫对猫头鹰猴生长的适应（Aotus nancymai）

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摘要

The purpose of this study was reactivation and adaptation of a strain of Plasmodium vivax to Aotus nancymai monkeys. A need arose for malarial parasites for use in serologic and molecular studies and for teaching slides. This particular strain of parasite had been characterized previously as producing high-density parasitemia in splenectomized New World monkeys and therefore represented a good candidate for reactivation. P. vivax (Vietnam II), isolated in 1970, was reactivated after adaptation in Aotus lemurinus griseimembra monkeys nearly 33 years earlier and adapted to A. nancymai monkeys. Passage was achieved by intravenous inoculation of parasite blood stages into splenectomized A. nancymai monkeys. Parasitemia was determined by analyzing daily blood smears stained with Giemsa. Maximum parasite counts ranged from 10,630 to 94,000 parasites/microl; the mean maximum parasite count for the four animals was 39,565 parasites/microl. Parasite counts of > 10,000/microl were maintained for 2 to 64 days. After only three passages of the parasite, attempts to reactive were successful. A. nancymai proved a suitable animal model for the recovery of this parasite. In conclusion, successful reactivation and adaptation of this parasite offers the capability to perform a series of diagnostic, immunologic, and molecular studies as well as to provide otherwise potentially unavailable teaching materials to healthcare professionals.
机译：这项研究的目的是使间日疟原虫菌株重新激活并适应南天牛。需要用于血清学和分子研究以及用于幻灯片的疟原虫。先前已将这种特定的寄生虫菌株表征为在脾切除的新大陆猴中产生高密度寄生虫病，因此代表了重新激活的良好候选者。间日疟原虫（越南II）分离于1970年，在适应了近33年的Aotus lemurinus griseimembra猴子中适应后被重新激活，并适应于A. nancymai猴子。通过将寄生虫血液阶段静脉内接种到脾切除的南希猴中来实现传代。通过分析每天用吉姆萨染色的血液涂片来确定寄生虫血症。最大寄生虫计数范围为10,630至94,000寄生虫/微克;四只动物的平均最大寄生虫计数为39565寄生虫/微克。寄生虫计数> 10,000 / microl保持2至64天。仅三遍寄生虫后，成功进行了反应。南希芽孢杆菌被证明是适合于该寄生虫恢复的动物模型。总之，成功地激活和适应这种寄生虫提供了进行一系列诊断，免疫和分子研究的能力，以及向医疗保健专业人员提供原本可能无法获得的教学材料的能力。

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