• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Comparative Medicine >Novel Technique for Retroperitoneal Implantation of Telemetry Transmitters for Physiologic Monitoring in Gottingen Minipigs (Sus scrofa domesticus)
【24h】

Novel Technique for Retroperitoneal Implantation of Telemetry Transmitters for Physiologic Monitoring in Gottingen Minipigs (Sus scrofa domesticus)

机译：腹膜后植入遥测变送器的新技术，用于哥廷根小型猪的生理监测

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Telemetric monitoring of physiologic parameters in animal models is a critical component of chemical and biologic agent studies. The long-term collection of neurobehavioral and other physiologic data can require larger telemetry devices. Furthermore, such devices must be implanted in a location that is safe, well-tolerated, and functional. Gottingen minipigs (Sus scrota domesticus) present an ideal large animal model for chemical agent studies due to their relatively small size, characterized health status, and ease of training and handling. We report an effective approach to implanting a novel device to measure transthoracic impedance to approximate respiratory tidal volume and rate in Suidae. We tested the approach using 24 male Gottingen minipigs. A ventral midline abdominal incision extending from the umbilicus to the prepuce was followed by a paramedian incision of the parietal peritoneum and dorsal blunt dissection to create a retroperitoneal pocket. The device was anchored inside the pocket to the internal abdominal musculature with 3-0 nonabsorbable suture, biopotential leads were routed through the abdominal musculature, and the pocket was closed with 3-0 absorbable suture. Paired biopotential leads were anchored intermuscularly at the level of the seventh rib midway between spine and sternum bilaterally to provide surrogate data for respiratory function. Postoperative recovery and gross pathology findings at necropsy were used to assess safety and refine the surgical procedure. Results demonstrated that this procedure permitted effective monitoring of complex physiologic data, including transthoracic impedance, without negatively affecting the health and behavior of the animals.
机译：遥测动物模型中的生理参数是化学和生物制剂研究的重要组成部分。长期收集神经行为和其他生理数据可能需要更大的遥测设备。此外，必须将此类设备植入安全，耐受良好且功能正常的位置。哥廷根小型猪（Sus scrota domesticus）由于其相对较小的尺寸，特定的健康状况以及易于训练和处理的特点，为化学制剂研究提供了理想的大型动物模型。我们报告了一种有效的方法，以植入一种新型设备来测量经胸阻抗，以近似Suidae的呼吸潮气量和呼吸速率。我们使用24只雄性哥廷根小型猪测试了该方法。从脐部延伸至包皮的腹中线腹部切口，然后腹膜旁正中切口和背侧钝器解剖，以形成腹膜后袋。用3-0不可吸收的缝合线将器械固定在口袋内部的内部腹部肌肉组织上，将生物电势导线穿过腹部肌肉组织，然后用3-0吸收性缝合线封闭口袋。配对的生物电势导线通过肌肉固定在双侧脊柱和胸骨之间的第七条肋骨的中间位置，以提供呼吸功能的替代数据。尸检后的术后恢复和总体病理检查结果可用于评估安全性和完善手术程序。结果表明，该程序可以有效监测复杂的生理数据，包括经胸阻抗，而不会对动物的健康和行为产生负面影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号