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Investigation of nanoparticles using magnetic resonance imaging after intravitreal injection

机译：玻璃体内注射后使用磁共振成像研究纳米颗粒

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Background: Magnetic nanoparticles may be used for focal delivery for cells, plasmids or drugs, and other applications. Here we asked whether magnetic nanoparticles could be detected in vivo at different time points after intravitreal injection by magnetic resonance imaging. Methods: Adult Sprague-Dawley rats received intravitreal injections of 50-nm or 4-μm magnetic particles into the left eye, with an equal volume of phosphate-buffered saline into the right eye (as controls). Animals were examined by magnetic resonance imaging at 1h, 1day and 5weeks after injection. Eyes, brain, liver, spleen and kidney were also imaged with high-resolution ex vivo magnetic resonance imaging scanning. Results: In vivo magnetic resonance imaging at the 1h and 1day time points more clearly detected magnetic particles in the 4μm group compared with the 50-nm group, although 50-nm magnetic nanoparticles were easily visualized with high-resolution magnetic resonance imaging ex vivo. Five weeks after intravitreal injection magnetic resonance imaging clearly detected 4-μm particles inside the eye, but by this time point the 50-nm magnetic nanoparticles could not be detected by either in vivo or ex vivo high-resolution magnetic resonance imaging. No magnetic particles were detected in any other organ. Conclusions: Magnetic resonance imaging could be used to track magnetic nanoparticles in the eye with the dosing selected for this study. Clearance varies by size, with 50-nm magnetic nanoparticles cleared more quickly than 4-μm particles. Thus, nanoparticles may provide advantages over micron-scale particles when considering risks associated with long-term persistence.
机译：背景：磁性纳米粒子可用于细胞，质粒或药物以及其他应用的焦点传递。在这里，我们询问是否可以通过磁共振成像在玻璃体内注射后的不同时间点在体内检测到磁性纳米粒子。方法：成年Sprague-Dawley大鼠在玻璃体内注射50 nm或4μm磁性颗粒到左眼，右眼注射等量的磷酸盐缓冲盐水（作为对照）。注射后1h，1天和5周通过磁共振成像检查动物。眼，脑，肝，脾和肾也通过高分辨率离体磁共振成像扫描成像。结果：与50nm组相比，在1h和1day时间点的体内磁共振成像更清楚地检测到4μm组中的磁性颗粒，尽管通过高分辨率离体磁共振成像可以轻松看到50nm磁性纳米颗粒。玻璃体内注射后五周，磁共振成像清楚地检测到了眼睛内部的4μm颗粒，但是到此时，体内或离体高分辨率磁共振成像都无法检测到50 nm磁性纳米颗粒。在任何其他器官中均未检测到磁性颗粒。结论：磁共振成像可用于跟踪眼中磁性纳米粒子的剂量，并为此研究选择剂量。间隙因尺寸而异，与4-μm颗粒相比，清除50 nm磁性纳米颗粒的速度更快。因此，当考虑与长期持久性有关的风险时，纳米粒子可以提供优于微米级粒子的优势。

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