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首页> 外文期刊>Biopreservation and biobanking >Developing a Predictive Tool for Reactive Oxygen Species Damage During Bovine Sperm Storage at Ambient Temperature
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Developing a Predictive Tool for Reactive Oxygen Species Damage During Bovine Sperm Storage at Ambient Temperature

机译：开发一种用于在室温下储存牛精子期间活性氧损伤的预测工具

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Preservation of sperm at ambient storage temperature (25 degrees C) provides a simplified alternative for short-term storage applications. The goals of the study were (i) to improve the storage ability of bovine sperm in liquid suspension by scavenging the reactive oxygen species (ROS) with the help of antioxidants and chelators, (ii) to develop a predictive tool to determine sperm motility as a function of storage temperature. Two types of antioxidants (catalase and superoxide dismutase, SOD) and 2 chelators (Desferal and ethylenediaminetetraacetic acid, EDTA) were used. Different concentrations of the supplements were added in egg yolk Tris (EYT) extender and stored at 4 degrees C, room temperature (25 degrees C), and 37 degrees C, respectively. Motility and membrane integrity (MI) were assessed on days 1, 4, 7, 10, and 14 for samples stored at 4 degrees C and 25 degrees C. Results did not suggest the beneficial effect of SOD and EDTA. Catalase caused a slight improvement in motility at 4 degrees C storage and its effectiveness was found to increase at higher storage temperature. The presence of Desferal resulted in a much higher increase in motility and membrane integrity at both 4 degrees C and 25 degrees C. Similarly, the fitting of Arrhenius damage model to the motility data provided a useful tool to predict motility degradation time as a function of storage temperature.
机译：在环境储存温度（25摄氏度）下保存精子可为短期储存应用提供简化的替代方法。该研究的目的是（i）在抗氧化剂和螯合剂的帮助下，通过清除活性氧（ROS）来提高牛精液在液体悬浮液中的储存能力;（ii）开发一种预测工具来确定精子的运动能力。储存温度的函数。使用了两种类型的抗氧化剂（过氧化氢酶和超氧化物歧化酶，SOD）和两种螯合剂（去铁和乙二胺四乙酸，EDTA）。将不同浓度的补充剂添加到蛋黄Tris（EYT）增量剂中，并分别存储在4摄氏度，室温（25摄氏度）和37摄氏度下。在4、25和25摄氏度下保存的样品在第1、4、7、10和14天评估了运动性和膜完整性（MI）。结果未表明SOD和EDTA的有益作用。过氧化氢酶在4摄氏度的储存条件下导致运动性略有改善，发现其有效性在较高的储存温度下会提高。 Desferal的存在导致在4摄氏度和25摄氏度时运动性和膜完整性的增加很多。类似地，将Arrhenius损伤模型与运动性数据拟合提供了一个有用的工具，可以预测运动性降解时间随温度的变化而变化。储存温度。

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