• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biopreservation and biobanking >Establishment and Characterization of Fibroblast Cell LineDerived from Siberian Tiger (Panthera tigris altaica)
【24h】

Establishment and Characterization of Fibroblast Cell LineDerived from Siberian Tiger (Panthera tigris altaica)

机译：西伯利亚虎（Panthera tigris altaica）成纤维细胞系的建立和表征

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The Siberian tiger ear marginal tissue fibroblast cell line (STF34) from 34 samples was successfully established using primary explants technique and cell cryoconservation technology. STF34 cells were adherent, with a population doubling time of 24 h. Chromosome analysis showed that 90.2%–91.6% of cells were diploid (2n=38). Isoenzyme analyses of lactate dehydrogenase and malate dehydrogenase showed that STF34 cells had no cross-contamination with other species. Tests for cell line contamination with bacteria, fungi, viruses, and mycoplasmas were all negative. Every index of the STF34 cell line meets all the standard quality controls of American Type Culture Collection. Not only has the germline of this important Siberian tiger species been preserved at the cell level, but also valuable material had been provided for genome, postgenome, and somacloning research.
机译：利用初级外植体技术和细胞冷冻保存技术成功建立了34个样本的西伯利亚虎耳边缘组织成纤维细胞系（STF34）。 STF34细胞是贴壁的，群体倍增时间为24 h。染色体分析显示90.2％–91.6％的细胞是二倍体（2n = 38）。乳酸脱氢酶和苹果酸脱氢酶的同工酶分析表明，STF34细胞与其他物种没有交叉污染。细胞系被细菌，真菌，病毒和支原体污染的测试均为阴性。 STF34细胞系的每个指标均符合美国典型培养物保藏中心的所有标准质量控制。不仅在细胞水平上保存了这种重要的西伯利亚虎物种的种系，而且还为基因组，后基因组和体细胞克隆研究提供了有价值的材料。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号