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Vitrification of Bovine Ovarian Tissue by the Solid-Surface Vitrification Method

机译：固体表面玻璃化法对牛卵巢组织进行玻璃化

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摘要

Exposure time and addition of sucrose to the vitrification medium as well as the solid-surface vitrification (SSV) on the morphology of bovine preantral follicles were evaluated. Ovarian tissue was exposed for 1, 5, or 10 min to 4.0 M ethylene glycol with or without the addition of 0.5 M sucrose. Subsequently, the tissue was washed out from cryoprotectants or vitrified by the SSV method. Independently of the presence of sucrose, exposure to vitrification solution for 10 min did reduce the percentages of normal follicles when compared with control. However, the highest rates of normal follicles were attained when tissue was previously exposed to the vitrification solution, with sucrose added or not, for 10 min. Although the SSV is a promising procedure to be applied in ovarian tissue, an optimal vitrification solution for bovine ovarian tissue needs to be developed.
机译：评价了牛前壁卵泡形态的暴露时间和蔗糖在玻璃化培养基中的添加以及固体表面玻璃化（SSV）。在有或没有添加0.5 M蔗糖的情况下，将卵巢组织暴露于4.0 M乙二醇中1、5或10分钟。随后，将组织从冷冻保护剂中洗出或通过SSV方法玻璃化。与蔗糖的存在无关，与对照相比，暴露于玻璃化溶液中10分钟确实降低了正常卵泡的百分比。但是，当组织事先暴露于玻璃化溶液中（添加或不添加蔗糖）达10分钟时，可获得最高的正常卵泡率。尽管SSV是在卵巢组织中应用的有前途的方法，但是需要开发用于牛卵巢组织的最佳玻璃化溶液。

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