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Age-Related Change in Phototaxis by Cercariae of Echinostoma caproni (Digenea: Echinostomatidae)

机译:卡氏棘皮动物尾rc尾Di的趋光性与年龄相关的变化(双生:Echinostomatidae)

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Cercarial dispersal is the result of fixed action patterns in response to reliable environmental cues. We tested the effect of age on the preference of Echinostoma caproni cercariae for light or dark. Individual cercariae were isolated within 10 min of release from Biomphalaria glabrata and placed in a Carolina? Deep-Well Slide. Half of the slide (top and bottom) was covered with electrical tape to exclude light. The entire chamber of the slide was observed on low power of a dissecting microscope so the cercaria was readily visible whenever it was in the lighted portion of the slide. The amount of time a cercaria spent in the light and the number of times it crossed from light to dark during a 5-min period at 0, 1,2, and 4 hr were determined (n = 20). The mean amount of time cercariae spent in the light declined significantly from immediately after release (127.7 sec) as compared to 1 hr (68.4 sec), 2 hr (51.6 sec), and 4 hr (10.6 sec) postemergence. The same pattern was seen in the average number of times cercariae crossed from light to dark in a 5-min period: 10.7, 7.2, 6.95, and 1.5, respectively. Cercariae showed no preference for light or dark immediately upon release (P = 0.119), nor was there a correlation between the amount of time spent in the light and the number of crossings at this time period. Cercariae spent a significantly greater amount of time in the dark with age (1-4 hr), and the number of crossings at each of these time periods was highly correlated with the time spent in the light. These findings suggest that light is not an important cue for E. caproni cercariae immediately upon release; however, they develop a strong preference for darker habitats, or an aversion to light, as they age.
机译:子宫颈扩散是响应可靠的环境提示而采取固定动作方式的结果。我们测试了年龄对浅色或深色的cap棘棘chin虫偏好的影响。在光滑小球藻释放后的10分钟内分离出单个尾c,并放置在卡罗来纳州。深度滑动。载玻片的一半(顶部和底部)被胶带覆盖以排除光线。在解剖显微镜的低功率下观察载玻片的整个腔室,因此无论何时在载玻片的亮部中都可以轻易看到尾c。确定在0、1、2和4小时的5分钟内,头孢子虫在光照下停留的时间以及从亮到暗的时间(n = 20)。与释放后的1小时(68.4秒),2小时(51.6秒)和4小时(10.6秒)相比,尾cer在光照中的平均时间从释放后立即(127.7秒)开始显着下降。在5分钟内,尾c从亮到暗的平均次数相同,分别为10.7、7.2、6.95和1.5。尾虫在释放后没有立即表现出对亮或暗的偏爱(P = 0.119),在亮处花费的时间与这段时间的穿越次数之间也没有相关性。尾虫在黑暗中随着年龄增长(1-4小时)花费的时间明显更多,并且每个时间段的穿越次数与光照时间高度相关。这些发现表明,光线并不是立即释放出的尾cap肠球菌的重要线索。然而,随着年龄的增长,它们对较暗的栖息地或对光的厌恶表现出强烈的偏爱。

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