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首页> 外文期刊>Biology of Reproduction: Offical Journal of the Society for the Study of Reproduction >Differential gene expression in ovaries of pregnant pigs with high and low prolificacy levels and identification of candidate genes for litter size.
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Differential gene expression in ovaries of pregnant pigs with high and low prolificacy levels and identification of candidate genes for litter size.

机译：高和低繁殖力水平的妊娠猪卵巢中的差异基因表达和产仔数候选基因的鉴定。

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摘要

Previous results from a genome scan in an F(2) Iberian x Meishan pig intercross showed several chromosome regions associated with litter size traits in this species. In order to identify candidate genes underlying these quantitative trait loci (QTL), we performed an ovary gene expression analysis during the sow's pregnancy. F(2) sows were ranked by their estimated breeding values for prolificacy: six sows with the highest estimated breeding value (EBV) (i.e., high prolificacy) and six sows with the lowest EBV (low prolificacy) were selected. Samples were hybridized using an Affymetrix GeneChip porcine genome array. Statistical analysis with a mixed model approach identified 221 differentially expressed probes, representing 189 genes. These genes were functionally annotated in order to identify genetic pathways overrepresented in this list. Among the functional groups most represented was, in first position, immune system response activation against external stimulus. The second group consisted of integrated genes that regulate maternal homeostasis by complement and coagulation cascades. A third group was involved in lipid and fatty acid enzymes of metabolic processes, which participate in the steroidogenesis pathway. In order to identify powerful candidate genes for prolificacy, the second approach of this study was to merge microarray data with the QTL positional information affecting litter size, previously detected in the same experimental cross. As a result, we have identified 27 differentially expressed genes colocalizing with QTL for litter size traits, which fulfill the biological, positional, and functional criteria.
机译：F（2）伊比利亚x梅山猪的杂交中的基因组扫描的先前结果显示，该物种中有几个与凋落物大小性状相关的染色体区域。为了确定这些定量性状基因座（QTL）的候选基因，我们在母猪怀孕期间进行了卵巢基因表达分析。 F（2）母猪按其繁殖力的估计育种值排名：选择了六只育种母猪的估计育种值（EBV）最高（即高繁殖力）和六只母猪的EBV最低（繁殖力低）。使用Affymetrix GeneChip猪基因组阵列杂交样品。使用混合模型方法进行的统计分析确定了221个差异表达的探针，代表189个基因。对这些基因进行了功能注释，以识别此列表中过多代表的遗传途径。在代表最多的功能组中，最重要的是针对外部刺激的免疫系统应答激活。第二组由整合基因组成，这些基因通过补体和凝血级联调节母体稳态。第三组涉及代谢过程的脂质和脂肪酸酶，其参与类固醇生成途径。为了鉴定功能强大的候选基因，本研究的第二种方法是将微阵列数据与影响同窝大小的QTL位置信息合并，该位置先前在同一实验杂交中检测到。结果，我们已经鉴定出27个与QTL共定位的凋落物大小性状的差异表达基因，这些基因满足生物学，位置和功能标准。

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