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首页> 外文期刊>Clinical and experimental pharmacology & physiology >Acid-sensing ion channels in trigeminal ganglion neurons innervating the orofacial region contribute to orofacial inflammatory pain
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Acid-sensing ion channels in trigeminal ganglion neurons innervating the orofacial region contribute to orofacial inflammatory pain

机译：三叉神经节神经元中支配口面区域的酸敏感离子通道有助于口面炎性疼痛

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摘要

Orofacial pain is a common clinical symptom that is accompanied by tooth pain, migraine and gingivitis. Accumulating evidence suggests that acid-sensing ion channels (ASICs), especially ASIC3, can profoundly affect the physiological properties of nociception in peripheral sensory neurons. The aim of this study is to examine the contribution of ASICs in trigeminal ganglion (TG) neurons to orofacial inflammatory pain. A Western blot (WB), immunofluorescence assay of labelled trigeminal ganglion neurons, orofacial formalin test, cell preparation and electrophysiological experiments are performed. This study demonstrated that ASIC1, ASIC2a and ASIC3 are highly expressed in TG neurons innervating the orofacial region of rats. The amplitude of ASIC currents in these neurons increased 119.72% (for ASIC1-like current) and 230.59% (for ASIC3-like current) in the formalin-induced orofacial inflammatory pain model. In addition, WB and immunofluorescence assay demonstrated a significantly augmented expression of ASICs in orofacial TG neurons during orofacial inflammation compared with the control group. The relative protein density of ASIC1, ASIC2a and ASIC3 also increased 58.82 ± 8.92%, 45.30 ± 11.42% and 55.32 ± 14.71%, respectively, compared with the control group. Furthermore, pharmacological blockade of ASICs and genetic deletion of ASIC1 attenuated the inflammation response. These findings indicate that peripheral inflammation can induce the upregulation of ASICs in TG neurons, causing orofacial inflammatory pain. Additionally, the specific inhibitor of ASICs may have a significant analgesic effect on orofacial inflammatory pain.
机译：口面部疼痛是常见的临床症状，伴有牙齿疼痛，偏头痛和牙龈炎。越来越多的证据表明，酸敏感离子通道（ASIC），尤其是ASIC3，可以深刻影响周围感觉神经元的伤害感受的生理特性。这项研究的目的是检查ASIC在三叉神经节（TG）神经元对口面部炎性疼痛的贡献。进行蛋白质印迹（WB），标记的三叉神经节神经元的免疫荧光测定，口面部福尔马林测试，细胞制备和电生理实验。这项研究表明ASIC1，ASIC2a和ASIC3在支配大鼠颌面区域的TG神经元中高表达。在福尔马林诱发的口腔炎症性疼痛模型中，这些神经元中ASIC电流的幅度增加了119.72％（对于ASIC1样电流）和230.59％（对于ASIC3样电流）。此外，与对照组相比，WB和免疫荧光分析表明在口腔炎症期间口腔TG神经元中ASIC的表达显着增加。与对照组相比，ASIC1，ASIC2a和ASIC3的相对蛋白密度也分别增加了58.82±8.92％，45.30±11.42％和55.32±14.71％。此外，ASIC的药理学阻断和ASIC1的基因缺失减弱了炎症反应。这些发现表明，外周炎症可以诱导TG神经元中ASIC的上调，从而引起口腔炎症性疼痛。此外，ASICs的特异性抑制剂可能对口腔炎性疼痛具有明显的镇痛作用。

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