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Influence of the Spacer of Cationic Gemini Amphiphiles on the Hydration of Lipoplexes

机译：阳离子双子座两亲物的间隔基对脂质体水合的影响

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摘要

The impact of the length of gemini surfactant spacer on complexation and condensation of calf thymus DNA by cationic mixed phospholipid/gemini liposomes was investigated by monitoring the conformational changes of DNA by circular dichroism and the lipid hydration level by the emission characteristics of the fluorescent probe laurdan included in the lipid bilayer. The length of the spacer was shown to influence, on one hand, the hydration level and the organization of the corresponding liposomes and, on the other, the variation of lipid hydration level and the DNA conformation upon complexation. In fact, in correspondence with the longest spacer we observed more hydrated liposomes, probably organized in domains, a higher extent of dehydration promoted by the addition of DNA, and a minor extent of DNA conformational change. The physicochemical features of lipoplexes were shown to depend on the [cationic headgroup]/[DNA single base] ratio.
机译：通过圆二色性监测DNA的构象变化以及通过荧光探针laurdan的发射特性监测脂质的水合作用水平，研究了双子表面活性剂间隔物长度对小牛胸腺DNA被阳离子混合磷脂/双子脂质体复合和缩合的影响。包括在脂质双层中。已显示间隔物的长度一方面影响水合水平和相应脂质体的组织，另一方面影响复合时脂质水合水平和DNA构象的变化。实际上，与最长的间隔子相对应，我们观察到了更多的水合脂质体，可能组织在域中，通过添加DNA促进了更高程度的脱水，以及次要的DNA构象变化。脂质体的理化特性显示取决于[阳离子头基] / [DNA单碱基]的比率。

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