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Diagnosis of malaria: a review of alternatives to conventional microscopy.

机译:疟疾的诊断:常规显微镜替代方法的综述。

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摘要

Malaria causes significant morbidity and mortality worldwide, including countries with mainly imported malaria. In developing nations, scarce resources lead to inadequate diagnostic procedures. In affluent countries, poor familiarity with malaria may cause clinical and laboratory misdiagnosis. Microscopy of Giemsa-stained thick and thin films remains the current standard for diagnosis. Although it has good sensitivity and allows species identification and parasite counts, it is time consuming, requires microscopical expertise and maintenance of equipment. Microscopy with fluorescent stains (QBC), dipstick antigen detection of HRP2 and pLDH (Parasight-F, ICT Malaria Pf, OptiMAL), polymerase chain reaction assays and some automated blood cell analysers offer new approaches and are reviewed here, with emphasis on clinical relevance and their potential to complement conventional microscopy, especially in countries with imported malaria.
机译:疟疾在包括主要是进口疟疾的国家在内的世界范围内引起大量发病和死亡。在发展中国家,稀缺资源导致诊断程序不足。在富裕国家,对疟疾的不熟悉可能会导致临床和实验室误诊。吉姆萨染色的厚膜和薄层的显微镜检查仍然是当前的诊断标准。尽管它具有良好的灵敏度并可以进行物种鉴定和寄生虫计数,但它很耗时,需要微观专业知识和设备维护。带有荧光染色剂(QBC)的显微镜检查,HRP2和pLDH的量油尺抗原检测(Parasight-F,ICT疟疾Pf,OptimAL),聚合酶链反应测定法和一些自动血细胞分析仪提供了新的方法,在此进行了综述,重点是临床相关性并具有补充传统显微镜的潜力,特别是在疟疾输入国。

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