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首页> 外文期刊>Clinical and laboratory haematology >Substantial changes in gene expression level due to the storage temperature and storage duration of human whole blood.
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Substantial changes in gene expression level due to the storage temperature and storage duration of human whole blood.

机译：基因表达水平的显着变化归因于人类全血的储存温度和储存时间。

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摘要

Blood is a valuable clinical sample for high-throughput analysis of gene expression and is likely to become more popular as a diagnostic tool and as a predictive measure of disease progression and drug responsiveness. Gene expression data from blood that has been stored at ambient temperature for greater than 1 h vs. blood samples that have been lysed immediately post-collection shows dramatic changes in relative gene expression for a number of cytokines, chemokines, and transcription factors. Results indicate significant changes in the relative expression of several genes, many of which were either up-regulated or down-regulated, because of storage at ambient temperature: (1) In only 4 h of storage at ambient temperature, greater than 10-fold increases in relative gene expression were observed for interleukin-8 (IL-8), c-myc, and c-fos; (2) Up-regulation of IL-8, a chemokine that mediates inflammatory cell migration, took place only 1-h after collection and increased nearly 100-fold by 4 h; (3) Down-regulation of several anti-inflammatory genes was observed for blood stored at ambient temperature; and (4) A general trend toward selective enhancement of inflammatory responses was observed, mediated by possible mRNA transcription and turnover. These results validate the need for the rapid lysis of whole blood after removal from the source.
机译：血液是用于基因表达高通量分析的有价值的临床样品，并且有可能作为诊断工具以及疾病进展和药物反应性的预测指标而变得越来越流行。与在采集后立即裂解的血液样品相比，在室温下存储超过1 h的血液中的基因表达数据显示，许多细胞因子，趋化因子和转录因子的相对基因表达发生了巨大变化。结果表明，几种基因的相对表达发生了显着变化，其中的许多基因由于在环境温度下的存储而被上调或下调：（1）在环境温度下仅4 h的存储，大于10倍白介素8（IL-8），c-myc和c-fos的相对基因表达增加; （2）介导炎症细胞迁移的趋化因子IL-8的上调仅在收集​​后1小时发生，并在4小时后增加近100倍; （3）在室温下保存的血液中观察到几种抗炎基因的下调; （4）观察到由可能的mRNA转录和更新介导的选择性增强炎症反应的总体趋势。这些结果证实了从源中取出后需要快速裂解全血。

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