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首页> 外文期刊>Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies >Hydrolysis of grapefruit peel waste with cellulase and pectinase enzymes
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Hydrolysis of grapefruit peel waste with cellulase and pectinase enzymes

机译：用纤维素酶和果胶酶水解葡萄柚皮废料

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摘要

Approximately 1 million metric tons of grapefruit were processed in the 2003/04 season resulting in 500,000 metric tons of peel waste. Grapefruit peel waste is usually dried, pelletized, and sold as a low-value cattle feed. This study tested different loadings of commercial cellulase and pectinase enzymes and pH levels to hydrolyze grapefruit peel waste to produce sugars. Pectinase and cellulase loadings of 0, 1, 2, 5, and 10 mg protein/g peel dry matter were tested at 45 degrees C. Hydrolyses were supplemented with 2.1 mg beta-glucosidase protein/g peel dry matter. Five mg pectinase/g peel dry matter and 2 mg cellulase/g peel dry matter were the lowest loadings to yield the most glucose. Optimum pH was 4.8. Cellulose, pectin, and hemicellulose in grapefruit peel waste can be hydrolyzed by pectinase and cellulase enzymes to monomer sugars, which can then be used by microorganisms to produce ethanol and other fermentation products. (c) 2006 Elsevier Ltd. All rights reserved.
机译：在2003/04年度，加工了约100万吨葡萄柚，造成500,000吨果皮废料。葡萄柚皮废料通常被干燥，制成颗粒并作为低价牛饲料出售。这项研究测试了商业纤维素酶和果胶酶的不同负载量和pH值水平，以水解葡萄柚皮废料产生糖。在45℃下测试0、1、2、5和10mg蛋白质/ g果皮干物质的果胶酶和纤维素酶装载量。水解物补充有2.1mgβ-葡糖苷酶蛋白质/ g果皮干物质。 5毫克果胶酶/克果皮干物质和2毫克纤维素酶/克果皮干物质的最低负载量可产生最多的葡萄糖。最适pH为4.8。葡萄果皮废料中的纤维素，果胶和半纤维素可以被果胶酶和纤维素酶水解为单体糖，然后微生物可以将其用于生产乙醇和其他发酵产物。 （c）2006 Elsevier Ltd.保留所有权利。

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