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首页> 外文期刊>日本化粧品技術者会誌 >Construction of a Three-Dimensional Human Skin Model Consisting of Keratinocytes, Dendritic Cells and Fibroblasts and Application of This Model for Alternative Animal Testing of Immune-Sensitizing Compounds Tadashi Uchino, Yoshiaki Ikarashi, Hiroshi
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Construction of a Three-Dimensional Human Skin Model Consisting of Keratinocytes, Dendritic Cells and Fibroblasts and Application of This Model for Alternative Animal Testing of Immune-Sensitizing Compounds Tadashi Uchino, Yoshiaki Ikarashi, Hiroshi

机译：由角质形成细胞，树突状细胞和成纤维细胞组成的三维人体皮肤模型的建立，以及该模型在免疫敏感化合物替代动物测试中的应用Tadashi Uchino，Yoshiaki Ikarashi，Hiroshi

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摘要

In order to establish in vitro evaluation of the sensitization of human skin, we attempted to make a three-dimensional human skin model consisting of three different cells, dendritic cells, keratinocytes and fibroblasts. The viability of the cells in the human skin model was observed after staining with hematoxylin and eosin. After 11-14-day incubation (horny layer was initially observed), the three-dimensional human skin model was used for experiments. Due to 2,4-dinitrochlorobenzene (DNCB) under a non-cytotoxic dose, the keratinocytes and dendritic cells in the human skin model significantly induced IL-4 release into the incubating medium and dendritic cells induced CD86 expression. On the other hand, with sodium dodecyl sulfate (SDS; non-sensitizer), the keratinocytes and dendritic cells did not significantly induce IL-4 release and the dendritic cells did not induce CD86 expression. The results suggested that this three-dimensional human skin model with dendritic cells could be applied as an alternative to animal testing of immune-sensitizing compounds.
机译：为了建立对人体皮肤敏化的体外评估，我们试图建立一个三维人体皮肤模型，该模型由三种不同的细胞，树突状细胞，角质形成细胞和成纤维细胞组成。用苏木精和曙红染色后，观察到人皮肤模型中细胞的活力。孵育11-14天后（最初观察到角质层），将三维人体皮肤模型用于实验。由于非细胞毒性剂量下的2,4-二硝基氯苯（DNCB），人皮肤模型中的角质形成细胞和树突状细胞显着诱导了IL-4释放到培养介质中，树突状细胞诱导了CD86表达。另一方面，使用十二烷基硫酸钠（SDS;非敏化剂）时，角质形成细胞和树突状细胞不会显着诱导IL-4释放，树突状细胞也不会诱导CD86表达。结果表明，这种具有树突状细胞的三维人体皮肤模型可以用作动物免疫敏感性化合物测试的替代方法。

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