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首页> 外文期刊>Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies >Enhanced hyaluronic acid production by a two-stage culture strategy based on the modeling of batch and fed-batch cultivation of Streptococcus zooepidemicus
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Enhanced hyaluronic acid production by a two-stage culture strategy based on the modeling of batch and fed-batch cultivation of Streptococcus zooepidemicus

机译：基于兽疫链球菌分批和补料分批培养模型的两阶段培养策略提高透明质酸生产

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摘要

This study aimed to enhance hyaluronic acid (HA) production by a two-stage culture strategy based on the modeling of batch and fed-batch culture of Streptococcus zooepidemicus. Batch culture had higher specific HA synthesis rate while fed-batch Culture had higher specific cell growth rate. The lower specific HA synthesis rate in fed-batch culture resulted from the competition of cell growth for the common precursors at a low substrate concentration. Based on the modeling of batch and fed-batch culture of S. zooepidemicus, a two-stage culture strategy was proposed to enhance HA production. S. zooepidemicus were cultured in a fed-batch mode with sucrose concentration maintained at 1,0 +/- 0.2 g/L during 0-8 h and then batch culture was performed during 8-20 h with an initial sucrose concentration of 15 g/L. With the proposed two-stage culture strategy, HA production was increased to 6.6 g/L compared with 5.0 g/ L in batch culture with the same total sucrose. The enhanced HA production by the proposed two-stage culture strategy resulted from the decreased inhibition of cell growth and the increased transformation rate of sucrose to HA. (c) 2008 Published by Elsevier Ltd.
机译：这项研究旨在通过基于兽疫链球菌分批和补料分批培养建模的两阶段培养策略来提高透明质酸（HA）的产量。分批培养具有较高的比HA合成速率，而补料分批培养具有较高的比细胞生长速率。分批补料培养中较低的HA合成速率较低是由于细胞生长竞争了低底物浓度下的常见前体。基于兽疫链球菌分批和补料分批培养的建模，提出了一种分两步培养的策略来提高HA的产量。兽疫链球菌在分批补料模式下培养，蔗糖浓度在0-8小时内保持在1,0 +/- 0.2 g / L，然后在8-20 h内进行分批培养，初始蔗糖浓度为15 g /升采用建议的两阶段培养策略，与总蔗糖相同的分批培养的HA产量相比，HA产量提高到6.6 g / L，而批量培养为5.0 g / L。拟议的两阶段培养策略提高了HA的产生，这是由于细胞生长抑制作用降低以及蔗糖向HA转化的速率增加。 （c）2008年由Elsevier Ltd.发布。

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