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首页> 外文期刊>Clinical and experimental allergy : >The influence of processing factors and non-atopy-related maternal and neonate characteristics on yield and cytokine responses of cord blood mononuclear cells.
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The influence of processing factors and non-atopy-related maternal and neonate characteristics on yield and cytokine responses of cord blood mononuclear cells.

机译：加工因素和与非动脉粥样硬化有关的母婴特征对脐血单核细胞产量和细胞因子反应的影响。

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RATIONALE: Several studies have evaluated the associations between cord blood cellular responses and atopic diseases in children, but the results of these studies are inconsistent. Variations in blood processing factors and maternal and infant characteristics are typically not accounted for and may contribute to these inconsistencies. METHODS: Cord blood samples were obtained from 287 subjects participating in the Childhood Origins of ASThma project, a prospective study of children at high risk for the development of asthma/allergies. Mononuclear cells were stimulated with phytohaemagglutinin (PHA), phorbal myristate acetate/ionomycin or a suspension of killed staphylococcus, and IFN-gamma, IL-10 and IL-13 were quantitated by ELISA. Cell yields and cytokine production were related to processing factors and maternal and infant characteristics. RESULTS: The strongest relationships between independent variables and cell yield or cytokine responses occurred with the season of birth. The highest median cell yields were seen in fall, and the lowest in summer (difference of 47%, P=0.0027). Furthermore, PHA-induced IL-5 and IL-13 responses were approximately 50% higher in spring and summer than in fall or winter (P<0.0001). Clots in the cord blood samples were associated with a reduced median cell yield (42% reduction, P<0.0001), and an increased PHA-induced IL-10 secretion (27% increase, P=0.004). CONCLUSIONS: These data suggest that season of collection, and to a lesser extent clotting in samples, affect cord blood mononuclear cell yield and cytokine responses. Careful documentation and analysis of processing and environmental variables are important in understanding biological relationships with cytokine responses, and also lead to greater comparability among studies using these techniques.
机译：理由：几项研究评估了儿童脐血细胞反应与特应性疾病之间的关联，但这些研究的结果不一致。血液处理因素以及母婴特征的变化通常无法解释，并且可能导致这些不一致。方法：从参加ASThma童年起源项目的287位受试者中获得脐带血样本，这是一项对患有哮喘/过敏高危儿童的前瞻性研究。用植物血凝素（PHA），肉豆蔻酸乙酸盐/离子霉素或杀死的葡萄球菌悬液刺激单核细胞，并通过ELISA定量IFN-γ，IL-10和IL-13。细胞产量和细胞因子产生与加工因子以及母婴特征有关。结果：自变量与细胞产量或细胞因子反应之间的最强关系与出生季节有关。在秋季可以看到最高的中值细胞产量，而在夏季则最低（相差47％，P = 0.0027）。此外，春季和夏季，PHA诱导的IL-5和IL-13反应比秋季或冬季高约50％（P <0.0001）。脐带血样品中的血块与中位数细胞产量降低（降低42％，P <0.0001）和PHA诱导的IL-10分泌增加（升高27％，P = 0.004）相关。结论：这些数据表明收集的季节和样品中的凝块程度较小，会影响脐带血单核细胞的产量和细胞因子的反应。仔细地记录和分析加工过程和环境变量对于理解与细胞因子反应的生物学关系非常重要，并且还导致使用这些技术的研究之间具有更大的可比性。

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