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首页> 外文期刊>Clinical and experimental allergy : >Characterization of IgE epitopes of Cuc m 2, the major melon allergen, and their role in cross-reactivity with pollen profilins.
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Characterization of IgE epitopes of Cuc m 2, the major melon allergen, and their role in cross-reactivity with pollen profilins.

机译：主要瓜变应原Cuc m 2的IgE表位的特征及其在与花粉蛋白水解酶交叉反应中的作用。

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BACKGROUND: Plant profilins are described as minor allergens, although with some exceptions in foods such as melon, watermelon or orange. In fact, they could be responsible for many cross-reactions among distantly related species. This is likely to be a consequence of the presence of common epitopes. OBJECTIVE: To characterize the B epitopes of Cuc m 2, a model of plant food profilin, using phage display techniques and to compare with other profilins, such as those of timothy grass and birch pollen, and human I profilin, to understand the mechanism of cross-reaction among members of this family. METHODS: IgE of melon-allergic patients was used to select clones from a phage display 12 mer peptide library. After two rounds of screening, Cuc m 2-specific clones were eluted and the DNA insertion sequenced. The residues of each clone were mapped on the Cuc m 2 surface to define a mimotope, which was also localized on the three-dimensional surfaces of other profilins. RESULTS: Seventeen melon-allergic patients were selected. Sera from each of them recognized the melon profilin, Cuc m 2, but the majority also recognized Phl p 12 or Bet v 2, timothy grass-, and birch-pollen profilins, respectively. A Cuc m 2 mimotope was defined and mapped onto its surface giving the following sequence: S(2)W(3)A(5)Y(6)D(9)H(10)T(111)P(112)G(113)Q(114)N(116)M(117)R(121)L(122). The homologous residues in Phl p 12 and Bet v 2 had almost identical sequences. By contrast, the homologous sequence in human profilin showed many differences. CONCLUSIONS: The identified mimotope could be involved in cross-reactions among food and pollen profilins. Many of these cross-reactions observed in the clinical realm could be explained by the presence of a common epitope found in food and pollen allergens. A new strategy of immunotherapy based on this IgE region could be used in alternative immunotherapy strategies.
机译：背景：植物蛋白原被描述为次要过敏原，尽管在诸如瓜，西瓜或橙子的食物中有一些例外。实际上，它们可能是造成远缘物种之间许多交叉反应的原因。这可能是由于常见表位的存在所致。目的：使用噬菌体展示技术表征植物性食物蛋白的模型Cuc m 2的B表位，并与其他蛋白（例如提摩太草和桦树花粉以及人I蛋白）进行比较，以了解其机理。这个家庭成员之间的交叉反应。方法：使用甜瓜过敏患者的IgE从噬菌体展示12聚体肽库中选择克隆。经过两轮筛选后，洗脱了Cuc m 2特异性克隆，并对DNA插入序列进行了测序。将每个克隆的残基定位在Cuc m 2表面上，以定义模拟表位，该表位也位于其他蛋白原的三维表面上。结果：选择了17例瓜类过敏患者。他们每个人的血清都识别出瓜蛋白纤维蛋白，Cuc m 2，但大多数人也分别认出了Phl p 12或Bet v 2，蒂莫西草和桦木花粉蛋白。定义了一个Cuc m 2模拟表位并将其映射到其表面，给出以下顺序：S（2）W（3）A（5）Y（6）D（9）H（10）T（111）P（112）G （113）Q（114）N（116）M（117）R（121）L（122）。在Phl p 12和Bet v 2中的同源残基具有几乎相同的序列。相比之下，人profilin中的同源序列显示出许多差异。结论：鉴定出的拟表位可能与食物和花粉蛋白的交叉反应有关。在临床领域中观察到的许多交叉反应可以通过在食物和花粉变应原中发现的常见表位的存在来解释。一种基于该IgE区的免疫治疗新策略可用于替代性免疫治疗策略。

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