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首页> 外文期刊>Clinical and experimental allergy : >Neonatal supplementation of processed supernatant from Lactobacillus rhamnosus GG improves allergic airway inflammation in mice later in life
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Neonatal supplementation of processed supernatant from Lactobacillus rhamnosus GG improves allergic airway inflammation in mice later in life

机译：鼠李糖乳杆菌GG的加工上清液的新生儿补充可改善小鼠晚年的过敏性气道炎症

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Background: Oral supplementation with probiotic bacteria can protect against the development of allergic and inflammatory diseases. Objective: The aim of this study was to investigate potential immunomodulatory and allergy-protective effects of processed Lactobacillus rhamnosus GG (LGG)-derived supernatants early in life in neonatal mice. Methods: In vitro, RAW264.7 mouse macrophages were stimulated with viable LGG, LGG-derived supernatants, prepared from different growth phases, and different size fractions thereof, and pro- and anti-inflammatory cytokine production was analysed. Supernatant fractions were also treated with protease, DNAse or carbohydrate-digesting enzymes to define the nature of immunomodulatory components. In vivo, neonatal Balb/c mice were orally supplemented with differentially processed LGG supernatants. Starting at 4 weeks of age, a protocol of ovalbumin-induced acute allergic airway inflammation was applied and protective effects of processed LGG supernatants were assessed. Results: Incubation of RAW264.7 cells with LGG-derived supernatants significantly increased TNF?? and IL-10 production. These effects were not restricted to a particular molecular size fraction. Treatment with protease, but not with DNAse or carbohydrate-digesting enzymes, completely abolished the immunomodulatory activities. Incubation of TLR/NOD-transfected cells with LGG-derived supernatants revealed that recognition and signalling of bioactive components is mediated by TLR2 and NOD2. In vivo supplementation of newborn mice with processed LGG-derived supernatants resulted in pronounced protective effects on the allergic inflammatory response as reflected by reduced eosinophil numbers, modified T helper cell cytokine production, significantly less lung inflammation and reduced goblet cell numbers in comparison with sham-treated controls. Conclusion: LGG-derived supernatants exert immunomodulatory activities, and neonatal administration of specifically processed supernatants may provide an alternative to viable probiotics in reducing allergic inflammatory responses. ? 2012 Blackwell Publishing Ltd.
机译：背景：口服益生菌可以预防过敏性和炎症性疾病的发展。目的：本研究的目的是研究新生鼠中鼠李糖乳杆菌GG（LGG）衍生的上清液的潜在免疫调节和过敏保护作用。方法：在体外，用存活的LGG，源自LGG的上清液刺激RAW264.7小鼠巨噬细胞，这些上清液由不同的生长期，不同的大小级分制备，并分析了促炎和抗炎细胞因子的产生。还用蛋白酶，DNAse或碳水化合物消化酶处理上清液部分，以定义免疫调节成分的性质。在体内，向新生的Balb / c小鼠口服补充了经过不同处理的LGG上清液。从4周龄开始，应用卵清蛋白诱导的急性过敏性气道炎症方案，并评估加工的LGG上清液的保护作用。结果：用LGG衍生的上清液温育RAW264.7细胞可显着增加TNFα。和IL-10生产。这些作用不限于特定的分子大小分数。用蛋白酶处理，而不用DNAse或碳水化合物消化酶处理，完全消除了免疫调节活性。用LGG衍生的上清液孵育TLR / NOD转染的细胞后发现，生物活性成分的识别和信号传导由TLR2和NOD2介导。与假手术组相比，在体内补充加工过的LGG来源的上清液对新生小鼠产生了对变应性炎症反应的显着保护作用，表现为嗜酸性粒细胞数量减少，T辅助细胞因子的产生改变，肺部炎症明显减少，杯状细胞数量减少。处理过的控件。结论：LGG衍生的上清液具有免疫调节活性，新生儿经特殊处理的上清液可为减少过敏性炎症反应提供可行的益生菌替代品。 ？ 2012布莱克威尔出版有限公司

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