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首页> 外文期刊>Clinical and experimental allergy : >Inhibitory effects of N-acetylcysteine on the functional responses of human eosinophils in vitro.
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Inhibitory effects of N-acetylcysteine on the functional responses of human eosinophils in vitro.

机译：N-乙酰半胱氨酸在体外对人嗜酸性粒细胞功能反应的抑制作用。

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BACKGROUND: Oxidative stress appears to be relevant in the pathogenesis of inflammation in allergic diseases like bronchial asthma. Eosinophils are oxidant-sensitive cells considered as key effectors in allergic inflammation. OBJECTIVE: The aim of this work was to study the effects of the clinically used antioxidant N-acetyl-L-cysteine (NAC) on the functional responses of human-isolated eosinophils. METHODS: Human eosinophils were purified from the blood of healthy donors by a magnetic bead separation system. The effects of NAC were investigated on the generation of reactive oxygen species (chemiluminescence and flow cytometry), Ca(2+) signal (fluorimetry), intracellular glutathione (GSH; flow cytometry), p47(phox)-p67(phox) translocation (Western blot) and eosinophil cationic protein (ECP) release (radioimmunoassay). RESULTS: NAC (0.1-1 mm) inhibited the extracellular generation of oxygen species induced by N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP) and eotaxin (in the presence of IL-5) with-logIC(50) values of 3.61+/-0.03 and 3.36+/-0.09, respectively. Also, the intracellular generation of hydrogen peroxide was virtually abolished by NAC (0.5-1 mm). NAC (1 mm) did not alter the fMLP-induced Ca(2+) signal but augmented the eosinophil content of reduced GSH and inhibited p47(phox)-p67(phox) translocation. NAC inhibited the release of ECP ( approximately 90% inhibition at 1 mm) from fMLP-activated eosinophils. CONCLUSION: Inhibition by NAC of human eosinophil functions in vitro is potentially useful in the treatment of allergic inflammation.
机译：背景：氧化应激似乎与支气管哮喘等过敏性疾病的炎症发病机制有关。嗜酸性粒细胞是对氧化剂敏感的细胞，被认为是过敏性炎症的关键效应物。目的：本研究的目的是研究临床上使用的抗氧化剂N-乙酰-L-半胱氨酸（NAC）对人分离的嗜酸性粒细胞功能反应的影响。方法：通过磁珠分离系统从健康供体的血液中纯化人嗜酸性粒细胞。研究了NAC对活性氧的产生（化学发光和流式细胞仪），Ca（2+）信号（荧光法），细胞内谷胱甘肽（GSH;流式细胞仪），p47（phox）-p67（phox）易位的影响（蛋白质印迹）和嗜酸性粒细胞阳离子蛋白（ECP）释放（放射免疫分析）。结果：NAC（0.1-1 mm）用logIC抑制了N-甲酰基-L-甲硫酰基-L-亮氨酰-L-苯丙氨酸（fMLP）和嗜酸细胞活化趋化因子（IL-5存在）诱导的氧种类的细胞外生成（50）的值分别为3.61 +/- 0.03和3.36 +/- 0.09。此外，NAC（0.5-1 mm）几乎消除了细胞内过氧化氢的生成。 NAC（1毫米）不会改变fMLP诱导的Ca（2+）信号，但增加了减少的GSH的嗜酸性粒细胞含量，并抑制了p47（phox）-p67（phox）易位。 NAC抑制了从fMLP激活的嗜酸性粒细胞释放ECP（在1毫米处抑制约90％）。结论：NAC在体外抑制人嗜酸性粒细胞功能可能对治疗过敏性炎症有用。

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