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首页> 外文期刊>Comparative Immunology, Microbiology and Infectious Diseases >Cloning and phylogenetic analysis of Interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) from Indian dromedaries (Camelus dromedarius)
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Cloning and phylogenetic analysis of Interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) from Indian dromedaries (Camelus dromedarius)

机译:印度单峰驼(Camelus dromedarius)白细胞介素6(IL-6)和肿瘤坏死因子-α(TNF-alpha)的克隆和系统发育分析

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摘要

The cDNAs of two proinflammatory cytokines viz., IL-6 and TNF-alpha from dromedarian camels were amplified by PCR using bactrian camel sequences and subsequently cloned for sequence analysis. Relationship based on amino acid revealed that dromedarian camel IL-6 shared 99.5% identity both at nucleotide and amino acid level with bactrian camel IL-6 and in case of TNF-alpha, the identity of dromedarian camel was 99.4% and 99.1% at nucleotide and amino acid level, respectively with that of bactrian camel. Phylogenetic analysis based on their amino acid sequences indicated the close relationship in these cytokine genes between dromedarian camel and other members of camelids
机译:使用双峰驼序列通过PCR扩增得自单峰骆驼的两种促炎细胞因子IL-6和TNF-α的cDNA,然后克隆用于序列分析。基于氨基酸的关系显示,单峰骆驼IL-6与双峰骆驼IL-6在核苷酸和氨基酸水平上均具有99.5%的同一性,而在TNF-α的情况下,单峰骆驼IL-6在核苷酸上的同一性为99.4%和99.1%和氨基酸水平,分别与双峰驼相近。根据它们的氨基酸序列进行的系统发育分析表明,这些细胞因子基因在单峰骆驼和其他骆驼科动物之间具有密切的关系

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