Sequence preference of mouse H1(0) and H1t.

Wellman SE; Song Y; Mamoon NM

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Sequence preference of mouse H1(0) and H1t.

机译：鼠标H1（0）和H1t的序列优先级。

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Histone H1 proteins bind to DNA and are important in formation and maintenance of chromatin structure. Little is known about differences among variant H1 histones in their interactions with DNA. We examined the effects of histones H1(0) and H1t on thermal denaturation of several DNA species. One of the DNA molecules was a 214-base-pair fragment from the plasmid pBR322, which contains an AT-rich and a GC-rich region. Both H1(0) and H1t bound preferentially to one region of the DNA fragment, a region that is relatively GC-rich. This result indicates that histones H1(0) and H1t are not totally nonspecific but rather bind with some sequence preference to DNA. This conclusion was supported by studies of other DNA species, including two 92-base-pair fragments derived from the two regions of the 214-mer, and several synthetic homocopolymers of DNA. Data obtained with the homocopolymers suggested that the binding preference was not simple preference for GC base pairs. The binding of the two H1 variants was not identical: there appear to be differences in binding site sizes, affinities, and sequence selectivities between H1t and H1(0).

机译：组蛋白H1蛋白与DNA结合，在染色质结构的形成和维持中很重要。关于变异H1组蛋白与DNA相互作用的差异知之甚少。我们检查了组蛋白H1（0）和H1t对几种DNA物种热变性的影响。 DNA分子之一是质粒pBR322的214个碱基对的片段，其中包含一个富含AT的区域和一个富含GC的区域。 H1（0）和H1t都优先绑定到DNA片段的一个区域，该区域是相对富含GC的区域。该结果表明组蛋白H1（0）和H1t不是完全非特异性的，而是与DNA具有一定的序列偏好性结合。该结论得到其他DNA种类研究的支持，其中包括两个来自214-mer两个区域的92个碱基对的片段，以及几种DNA的合成均聚物。用均聚物获得的数据表明，结合偏好不是GC碱基对的简单偏好。两个H1变体的结合并不完全相同：H1t和H1（0）之间的结合位点大小，亲和力和序列选择性似乎有所不同。

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《Biochemistry》 |1999年第40期|共7页
作者
Wellman SE; Song Y; Mamoon NM;
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正文语种 eng
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DNA-Binding Proteins; Histones; nuclear protein H1(o); Oligodeoxyribonucleotides; DNA结合蛋白质类; 组蛋白类;

机译：DNA-Binding Proteins;Histones;nuclear protein H1(o);Oligodeoxyribonucleotides;DNA结合蛋白质类;组蛋白类;

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1. Sequence preference of mouse H1(0) and H1t. [J] . Wellman SE, Song Y, Mamoon NM Biochemistry . 1999,第40期

机译：鼠标H1（0）和H1t的序列优先级。
2. MQ-HCN-based pulse sequences for the measurement of (13)C1 '-(1)H1 ', (13)C1 '-N-15, (1)H1 '-N-15, (13)C1 '-(13)C2 ', (1)H1 '-(13)C2 ', (13)C6/8-(1)H6/8, (13)C6/8-N-15, (1)H6/8-N-15, (13)C6-(13)C5, (1)H6-(13)C5 dipolar couplings in C-13, N-15-labeled [J] . Yan JL., Corpora T., Pradhan P., Journal of Biomolecular NMR . 2002,第1期

机译：基于MQ-HCN的脉冲序列，用于测量（13）C1'-（1）H1'，（13）C1'-N-15，（1）H1'-N-15，（13）C1'-（ 13）C2'，（1）H1'-（13）C2'，（13）C6 / 8-（1）H6 / 8，（13）C6 / 8-N-15，（1）H6 / 8-N -15，C-13，N-15标记的（13）C6-（13）C5，（1）H6-（13）C5双极偶合
3. Nucleotide sequences of H1 histone genes from Xenopus laevis. A recently diverged pair of H1 genes and an unusual H1 pseudogene [J] . H.R. Woodland, P.C. Turner, R.W. Old, Nucleic acids research . 1983,第12期

机译：非洲爪蟾H1组蛋白基因的核苷酸序列。一对最近分离的H1基因对和一个不寻常的H1假基因
4. Identification of Tranilast-Binding Proteins from Mouse Xenograft Model with Human and Mouse Genome Sequence Information [C] . Noriyuki Inomata, Takashi Shinkawa, Kohji Nagano, ASMS Conference on Mass Spectrometry and Allied Topics . 2008

机译：用人和小鼠基因组序列信息鉴定小鼠异种移植模型的旋转蛋白结合蛋白
5. Causes and consequences of male and female partner preference in the monogamous oldfield mouse (Peromyscus polionotus subgriseus). [D] . Good, Tatjana C. 2004

机译：一夫一妻制的老田鼠（Peromyscus polionotus subgriseus）中男性和女性伴侣偏爱的原因和后果。
6. Nucleotide sequences of H1 histone genes from Xenopus laevis. A recently diverged pair of H1 genes and an unusual H1 pseudogene. [O] . P C Turner, T C Aldridge, H R Woodland, 1983

机译：非洲爪蟾H1组蛋白基因的核苷酸序列。一对最近分离的H1基因和一个不寻常的H1假基因。
7. Ca2+-binding proteins in the retina: from discovery to etiology of human disease11The nucleotide sequences reported in this manuscript have been submitted to the GenBank™/EMBL databank with the following accession numbers: short form of human CaBP1, AF169148; long form of human CaBP1, AF169149; short form of bovine CaBP1, AF169150; long form of bovine CaBP1, AF169151; short form of mouse CaBP1, AF169153; long form of mouse CaBP1, AF1691152; human CaBP2, AF169154; bovine CaBP2, AF169155; short form of mouse CaBP2, AF169156; long form of mouse CaBP2, AF169157; human CaBP3, AF169158; human CaBP5, AF169159; bovine CaBP5, AF169160; mouse CaBP5, AF169161; human CaBP2 genomic sequence, AF170811; exons 1, 2-3-4, 5, 6 of human CaBP5 genomic sequence, AF170812, AF170813, AF170814, AF170815, respectively, and exons 1-2, 3-4, 5, 6 of human CaBP3 genomic sequence, AF170816, AF170817, AF170818, AF170815, respectively, and published recently F. Haeseleer et al. (2000) J. Biol. Chem. 275, 1247–1260. [O] . Sokal Izabela, Li Ning, Verlinde Christophe L.M.J., 2000

机译：视网膜中的Ca2 +结合蛋白：从人类疾病的发现到病因学11该手稿中报道的核苷酸序列已提交至GenBank™/ EMBL数据库，其登录号如下：人CaBP1的缩写形式，AF169148；人类CaBP1的长形式，AF169149；牛CaBP1的缩写，AF169150；牛CaBP1的长形式，AF169151；小鼠CaBP1，AF169153的缩写；长型小鼠CaBP1，AF1691152；人CaBP2，AF169154；牛CaBP2，AF169155;小鼠CaBP2的缩写，AF169156；长型小鼠CaBP2，AF169157；人CaBP3，AF169158；人CaBP5，AF169159；牛CaBP5，AF169160;小鼠CaBP5，AF169161；人CaBP2基因组序列，AF170811；人CaBP5基因组序列的外显子1、2-3-4、5、6，AF170812，AF170813，AF170814，AF170815和人CaBP3基因组序列的外显子1-2、3-4、5、6，AF170816，AF170817 ，AF170818，AF170815并在最近发表F. Haeseleer等。（2000）J.Biol。化学275，1247–1260。

Sequence preference of mouse H1(0) and H1t.

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