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首页> 外文期刊>Biochemistry >Sterols and Sphingolipids Strongly Affect the Growth of Fusion Pores Induced by the Hemagglutinin of Influenza Virus
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Sterols and Sphingolipids Strongly Affect the Growth of Fusion Pores Induced by the Hemagglutinin of Influenza Virus

机译：甾醇和鞘脂强烈影响流感病毒血凝素诱导的融合孔的生长

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Cells expressing the hemagglutinin (HA) of influenza virus were fused to planar phospholipid bilayer membranes to evaluate the effects of sterols and sphinglipids in the target bilayer membranes on properties of fusion pores.Typically,in the absence of sterol,flickering pores are observed,followed by a successfull pore (i.e.,a pore that fully opens).The incorporation of cholesterol into the lipid bilayer had a marked effect: it greatly decreased the number of flickers,and the first pore formed was usually successful.Similar effects were groduced by the sterols epicholesterol and 5#beta#-cholestanol.In contrast,the sterols cholesteryl acetate,coprostanol,and stanolone did not affect pore flickering,and a successful pore was observed to follow that the 3-OH of cholesterol is essential to reduce flickering,but it does not matter if the 3-OH is in an #alpha# or #beta# configuration.The double bond is also not critical for the actions of cholesterol nor is the fact that it is a flat molecule.The sphingolipids sphingomyelin,lactosyl cereboside, and glucosyl cerebroside tended to inhibit full pore enlargement,prologning the stage of pore flickering.If a sphingolipid and a sterol that strongly interact were both included in the planar membrane,the pattern of flickering was the same as if neither had been included in the bilayer.However,of a sphingolipid and sterol the do not interact with each other were included in the bilayer,the reduced flickering characteristic of the sterol was observed.
机译：将表达流感病毒血凝素（HA）的细胞融合到平面磷脂双层膜上，以评估目标双层膜中的固醇和鞘脂对融合孔性能的影响。通常，在没有固醇的情况下，观察到闪烁的孔，随后通过成功的毛孔（即完全打开的毛孔）。将胆固醇掺入脂质双层中具有显着的作用：它大大减少了闪烁的数量，并且第一个形成的毛孔通常是成功的。固醇表胆固醇和5＃β＃-胆固醇。相反，固醇乙酸胆固醇，coprostanol和甾烷酮不会影响毛孔闪烁，并且观察到成功的毛孔表明胆固醇的3-OH对于减少闪烁是必不可少的，但是3-OH处于＃alpha＃或＃beta＃构型无关紧要。双键对于胆固醇的作用也不重要，也不是平面分子的事实。 e。鞘脂鞘磷脂，乳糖苷脑苷和葡糖脑苷倾向于抑制毛孔的完全扩张，延长毛孔闪烁的阶段。如果在平面膜中同时包含了鞘脂和固醇，并且两者相互作用强烈，则闪烁的模式是相同的但是，在鞘脂和固醇彼此不相互作用的情况下，在双层中包含固醇的闪烁特性降低。

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