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首页> 外文期刊>Biochemistry >Reaction of hydrogen peroxide with ferrylhemoglobin: Superoxide production and heme degradation
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Reaction of hydrogen peroxide with ferrylhemoglobin: Superoxide production and heme degradation

机译：过氧化氢与亚铁血红蛋白的反应：超氧化物的产生和血红素的降解

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The reaction of Fe(II) hemoglobin (Hb) but not Fe(III) hemoglobin (metHb) with hydrogen peroxide results in degradation of the heme moiety. The observation that heme degradation was inhibited by compounds, which react with ferrylHb such as sodium sulfide, and peroxidase substrates (ABTS and o-dianisidine), demonstrates that ferrylHb formation is required for heme degradation. A reaction involving hydrogen peroxide and ferrylHb was demonstrated by the finding that heme degradation was inihibited by the addition of catalase which removed hydrogen peroxide even after the maximal level of ferrylHb was reached. The reaction of hydrogen peroxide with ferrylHb to produce heme degradation products was shown by electron paramagnetic resonance to involve the one-electron oxidation of hydrogen peroxide to the oxygen free radical, superoxide. The inhibition by sodium sulfide of both superoxide production and the formation of fluorescent heme degradation products links superoxide production with heme degradation. The inability to produce heme degradation products by the reaction of metHb with hydrogen peroxide was explained by the fact that hydrogen peroxide reacting with oxoferrylHb undergoes a two-electron oxidation, producing oxygen instead of superoxide. This reaction does not produce heme degradation, but is responsible for the catalytic removal of hydrogen peroxide. The rapid consumption of hydrogen peroxide as a result of the metHb formed as an intermediate during the reaction of reduced hemoglobin with hydrogen peroxide was shown to limit the extent of heme degradation. [References: 70]
机译：Fe（II）血红蛋白（Hb）但非Fe（III）血红蛋白（metHb）与过氧化氢的反应导致血红素部分降解。观察到血红素的降解被与硫化氢等ferrylHb反应的化合物和过氧化物酶底物（ABTS和o-dianisidine）抑制，表明血红素降解需要形成ferrylHb。通过发现过氧化氢酶抑制了血红素降解，该过氧化氢和过氧化氢与ferrylHb的反应被抑制，即使过了最高水平的过氧化氢酶也去除了过氧化氢。电子顺磁共振显示，过氧化氢与ferrylHb产生血红素降解产物的反应涉及将过氧化氢单电子氧化为氧自由基超氧化物。硫化钠对超氧化物产生的抑制和荧光血红素降解产物的形成将超氧化物的产生与血红素降解联系在一起。 metHb与过氧化氢反应无法产生血红素降解产物的解释是，过氧化氢与oxoferrylHb反应会经历两电子氧化，从而生成氧气而不是超氧化物。该反应不会产生血红素降解，但负责催化去除过氧化氢。由于在还原的血红蛋白与过氧化氢反应过程中作为中间体生成的metHb，过氧化氢的快速消耗限制了血红素的降解程度。 [参考：70]

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