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首页> 外文期刊>Biochemistry >Electron transport routes in whole cells of synechocystis sp. strain PCC 6803: the role of the cytochrome bd-type oxidase
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Electron transport routes in whole cells of synechocystis sp. strain PCC 6803: the role of the cytochrome bd-type oxidase

机译：突触藻全细胞中的电子传输途径。 PCC 6803菌株：细胞色素bd型氧化酶的作用

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摘要

The plastoquinone pool is the central switching point of both respiratory and photosynthetic electron transport in cyanobacteria. Its redox state can be monitored noninvasively in whole cells using clorophyll fluorscence induction, avoiding possible artifacts associated with thylakoid membrane preparations. This method was applied to cells to Synechocystic sp. PCC 6803 to study respiratory reactions involving the pastoquinone pool. The role of the respiratory oxidases known from the genomic sequence of Synechocystis sp. PCC 6803 was investigated by a combined strategy using inhibitors and deletion strains that lack one or more of these oxidases. The putative quinol oxidase of the cytochrome bd-type was shown to participate in electron transport in thylakoid membranes. The activity of this enzyme in thylakoids was strongly dependent on culture conditions; it was increased under conditions where the activity of the cytochrome b_6f complex alone amy be insufficient for preventing over-reduction of the PQ pool. In contrast, no indication of quinol oxidase activity in thylakoids was found for a second alternative oxidase encoded by the ctall genes.
机译：质体醌库是蓝细菌中呼吸和光合电子传递的中心转换点。可以使用叶绿素荧光诱导在全细胞中无创监测其氧化还原状态，避免可能与类囊体膜制备有关的伪影。该方法被应用于细胞囊藻。 PCC 6803用于研究涉及Pastoquinone库的呼吸系统反应。呼吸氧化酶的作用从Synechocystis sp。的基因组序列已知。通过组合策略研究了PCC 6803，该策略使用的抑制剂和缺失菌株缺乏一种或多种这些氧化酶。已证明细胞色素bd型推定的喹诺酚氧化酶参与类囊体膜的电子传递。该酶在类囊体中的活性强烈依赖于培养条件。在单独的细胞色素b_6f复合物的活性不足以防止PQ池过度减少的条件下，它增加了。相比之下，没有发现类囊体中喹诺酮氧化酶活性的迹象表明由ctall基因编码的第二种替代氧化酶。

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