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首页> 外文期刊>Biochemistry >YidC Is Strictly Required for Membrane Insertion of Subunits and c of the F_1F_0ATP Synthase and SecE of the SecYEG Translocase
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YidC Is Strictly Required for Membrane Insertion of Subunits and c of the F_1F_0ATP Synthase and SecE of the SecYEG Translocase

机译：膜插入亚基和c的F_1F_0ATP合酶和SecYEG易位酶的SecE严格需要YidC

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YidC was previously discovered to play a critical role for the insertion of the Sec-independent M13 procoat and Pf3 coat phage proteins into the Escherichia coli inner membrane. To determine whether there is an absolute requirement of YidC for membrane protein insertion of any endogenous E. coli proteins, we investigated a few representative membrane proteins. We found that membrane subunits of the F_0 sector of the F_1F_0ATP synthase and the SecE protein of the SecYEG translocase are highly dependent on YidC for membrane insertion, based on protease mapping and immunoblot analysis. We found that the SecE dependency on YidC for membrane insertion does not contradict the observation that depletion of YidC does not block SecYEG-dependent protein export at 37 deg C. YidC depletion does not decrease the SecE level low enough to block export at 37 deg C. In contrast, we found that protein export of OmpA is severely blocked at 25 deg C when YidC is depleted, which may be due to the decreased SecE level, as a 50% decrease in the SecE levels drastically affects protein export at the cold temperature [Schatz, P.J., Bieker, K.L., Ottemann, K.M., Silhavy, T.J., and Beckwith, J. (1991) EMBO J. 10, 1749-57]. These studies reported here establish that physiological substrates of YidC include subunits of the ATP synthase and the SecYEG translocase, demonstrating that YidC plays a vital role for insertion of endogenous membrane proteins in bacteria.
机译：以前发现，YidC对于将不依赖于Sec的M13前涂层和Pf3外膜噬菌体蛋白插入大肠杆菌内膜起着关键作用。为了确定是否绝对需要将YidC插入任何内源性大肠杆菌蛋白的膜蛋白中，我们研究了一些代表性的膜蛋白。我们发现，基于蛋白酶图谱和免疫印迹分析，F_1F_0ATP合酶的F_0区段的膜亚基和SecYEG转位酶的SecE蛋白高度依赖于YidC进行膜插入。我们发现SecE对YidC的膜插入依赖性并不与YidC的耗竭不会阻止SecYEG依赖的蛋白质在37摄氏度出口的观察结果相矛盾。YidC的耗竭不会降低SecE的水平，不足以阻止在37摄氏度的出口。相比之下，我们发现，当YidC耗尽时，OmpA的蛋白质输出在25摄氏度时会严重受阻，这可能是由于SecE水平降低所致，因为在低温下SecE水平降低50％会严重影响蛋白质输出[Schatz，PJ，Bieker，KL，Ottemann，KM，Silhavy，TJ和Beckwith，J.（1991）EMBO J. 10，1749-57]。这些研究报告表明，YidC的生理底物包括ATP合酶和SecYEG转移酶的亚基，表明YidC在细菌中内源膜蛋白的插入中起着至关重要的作用。

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